Caenorhabditis elegans UNC-98, a C2H2Zn finger protein, is a novel partner of UNC-97/PINCH in muscle adhesion complexes
Caenorhabditis elegans UNC-98, a C2H2Zn finger protein, is a novel partner of UNC-97/PINCH in muscle adhesion complexes
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DOI：
10.1091/mbc.e02-10-0676
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发表时间：
2003-06-01
影响因子：
3.3
通讯作者：
Benian, GM
中科院分区：
文献类型：
--
作者：
Mercer, KB;Flaherty, DB;Benian, GM
为了进一步了解肌肉收缩装置的组装和维持，我们在秀丽隐杆线虫的肌肉中鉴定了一种新蛋白质，即α-98。UNC-98突变体显示出运动性降低和肌肉结构的特征性缺陷。我们发现，突变肌肉的主要缺陷是在M线和致密体（Z线类似物）。在功能和组成上，线虫M线和致密体类似于非肌肉细胞的局灶性粘连。UNC-98是一种新型310个残基的多肽，由四个C2 H2锌指和几个可能的核定位信号和核输出信号序列组成。通过使用α-98抗体和绿色荧光蛋白融合物（与全长α-98和α-98片段融合），我们已经证明α-98存在于M线、肌细胞核和可能存在于致密体。此外，我们证明了1）N-末端106个氨基酸是核定位所必需的，也是足够的，2）C-末端（第四）锌指是定位到葡萄酒和致密体所必需的。C.98与C.97相互作用，产生一个C. PINCH的elegans同系物。我们提出，α-98既是肌肉局灶性粘连的结构成分，也是影响基因表达的核蛋白。
To further understand the assembly and maintenance of the muscle, contractile apparatus, we have identified a new protein, UNC-98, in the muscle of Caenorhabditis elegans. unc-98 mutants display reduced motility and a characteristic defect in muscle structure. We show that the major defect in the mutant muscle is in the M-lines and dense, bodies (Z-Iine analogs). Both functionally and compositionally, nematode M-lines and dense bodies are analogous to focal adhesions of nonmuscle cells. UNC-98 is a novel 310-residue polypeptide consisting of four C2H2 Zn fingers and several possible nuclear localization signal and nuclear export signal sequences. By use of UNC-98 antibodies and green fluorescent protein fusions (to full-length UNC-98 and UNC-98 fragments), we have shown that UNC-98 resides at M-lines, muscle cell nuclei, and possibly at dense bodies. Furthermore, we demonstrated that 1) the N-terminal 106 amino adds are both necessary and sufficient for nuclear localization, and 2) the C-terminal (fourth) Zn finger is required for localization to Wines and dense bodies. UNC-98 interacts with UNC-97, a C. elegans homolog of PINCH. We propose, that UNC-98 is both a structural component of muscle focal adhesions and a nuclear protein that influences gene expression.