Paracellular Barrier and Tight Junction Protein Expression in the Immortalized Brain Endothelial Cell Lines bEND.3, bEND.5 and Mouse Brain Endothelial Cell 4
Paracellular Barrier and Tight Junction Protein Expression in the Immortalized Brain Endothelial Cell Lines bEND.3, bEND.5 and Mouse Brain Endothelial Cell 4
复制标题
DOI：
10.1248/bpb.b12-00915
复制
发表时间：
2013-03-01
影响因子：
2
通讯作者：
Kataoka, Yasufumi
中科院分区：
文献类型：
--
作者：
Watanabe, Takuya;Dohgu, Shinya;Kataoka, Yasufumi
血脑屏障（BBB）是由脑内皮细胞形成的。已经建立了许多永生化脑内皮细胞系;这些细胞系已被用作体外BBB模型。本研究的目的是评估永生化小鼠脑内皮细胞系bEND.3，bEND.5细胞，小鼠脑内皮细胞4（MBEC 4），以及原代小鼠脑内皮细胞pMBEC的细胞旁屏障特性。bEND.3细胞显示出与pMBEC相似的对荧光素钠的低渗透性和紧密连接蛋白（claudin-5、occludin和ZO-1）的明显染色; MBEC 4和bEND.5细胞的这些屏障特性低。此外，bEND.3细胞在所有细胞中表达最高水平的密蛋白-5。这些结果表明，bEND.3细胞是一个方便和有用的模型，用于评估血脑屏障功能，特别是细胞旁屏障。
The blood brain barrier (BBB) is formed by brain endothelial cells. Many immortalized brain endothelial cell lines have been established; these have been used as in vitro BBB models. The aim of the present study was to assess the paracellular barrier properties of the immortalized mouse brain endothelial cell lines bEND.3, bEND.5 cells, and mouse brain endothelial cell 4 (MBEC4), and those of the primary mouse brain endothelial cells pMBECs. bEND.3 cells showed low permeability to sodium fluorescein and obvious staining of tight junction proteins (claudin-5, occludin and ZO-1) similar to pMBECs; these barrier properties of MBEC4 and bEND.5 cells were low. In addition, bEND.3 cells expressed the highest level of claudin-5 among all cells. These results suggest that bEND.3 cells are a convenient and useful model for evaluating BBB function, especially the paracellular barrier.