Regulation of pancreatitis-associated protein (HIP/PAP) mRNA levels in mouse pancreas and small intestine.
Regulation of pancreatitis-associated protein (HIP/PAP) mRNA levels in mouse pancreas and small intestine.
复制标题
小鼠胰腺和小肠中胰腺炎相关蛋白 (HIP/PAP) mRNA 水平的调节。
DOI：
10.1042/cs0910213
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发表时间：
1996
期刊：
影响因子：
6
通讯作者：
Farzin Farzaneh
中科院分区：
文献类型：
--
作者：
A. Mckie;Robert J. Simpson;Simon Ghosh;Timothy J. Peters;Farzin Farzaneh
1. 在本研究中，我们检测了胰腺炎相关蛋白mRNA在小鼠胰腺和小肠中的表达，并确定了一些因素对RNA稳态水平的影响。2. 胰腺中正常低水平的胰腺炎相关蛋白mRNA在缺氧6小时后增加了数倍，在缺氧24小时后达到峰值（约为正常水平的10倍）。缺氧3天后，胰腺炎相关蛋白mRNA水平再次检测不到。3. 在胰腺中，胰腺炎相关蛋白mRNA水平也因酒精和铁超载而升高，但扑热息痛不升高。4. 在小肠中，胰腺炎相关蛋白mRNA在正常回肠中的表达高于十二指肠。在回肠中，缺氧6 h后胰腺炎相关蛋白mRNA水平升高7 ~ 15倍，24 h达到峰值，缺氧3 d后水平下降，但仍高于正常水平。5. 在回肠中，与对照组相比，长期（4周）饮食铁缺乏降低了胰腺炎相关蛋白mRNA水平，而肠外铁超载增加了胰腺炎相关蛋白mRNA水平。6. 这些数据表明，氧张力和铁状态对胰腺炎相关蛋白基因表达的调节。
1. In the present study we have examined the expression of pancreatitis-associated protein mRNA in mouse pancreas and small intestine and determined the effect of a number of factors on the steady-state level of the RNA. 2. The normally low level of pancreatitis-associated protein mRNA in pancreas increased severalfold after 6 h of hypoxia, reaching peak levels (approximately 10-fold greater than normal) after 24 h hypoxia. After 3 days' hypoxia pancreatitis-associated protein mRNA levels were again undetectable. 3. In the pancreas the level of pancreatitis-associated protein mRNA was also increased by alcohol and iron overload, but not by paracetamol. 4. In the small intestine expression of pancreatitis-associated protein mRNA was higher in normal ileum than in duodenum. In the ileum pancreatitis-associated protein mRNA levels were increased 7 to 15-fold after 6 h hypoxia, reaching peak levels by 24 h. Levels declined after 3 days' hypoxia, but remained higher than normal. 5. In the ileum long-term (4 weeks) dietary iron deficiency reduced pancreatitis-associated protein mRNA levels compared with control fed mice, whereas parenteral iron overload increased pancreatitis-associated protein mRNA levels. 6. The data presented suggest regulation of pancreatitis-associated protein gene expression by both oxygen tension and iron status.