Measurement of cytokine and adhesion molecule expression in synovial tissue by digital image analysis
Measurement of cytokine and adhesion molecule expression in synovial tissue by digital image analysis
复制标题
DOI：
10.1136/ard.60.3.296
复制
发表时间：
2001-03-01
影响因子：
27.4
通讯作者：
Tak, PP
中科院分区：
文献类型：
--
作者：
Kraan, MC;Smith, MD;Tak, PP
目的：数字图像分析(DIA)为免疫组织化学检测滑膜组织(ST)提供了定量染色面积和染色强度的机会。本研究旨在比较DIA和半定量分析(SQA)的敏感性。方法收集10例活动期类风湿关节炎(RA)患者膝关节标本，并于临床完全缓解后进行随访。10例非炎症性膝痛患者的ST样本作为对照。以3-氨基-9-乙基咔唑(AEC)或对二乙氨基苯甲醛(DAB)为染料，采用免疫组织化学方法，用抗白介素1-β(IL-1β)和血管细胞黏附分子-1(VCAM-1)的抗体进行免疫组织化学染色。对所有截面进行半定量分析(0-4)和最大60个高功率场(HPF)的DIA。平均积分光密度计算为染色面积(经总组织面积校正)和光密度的乘积。结果：SQA和DIA都能够评估活动期PA的ST、缓解期RA和对照组之间IL1β和VCAM-1的表达差异。SqA和DIA具有极好的相关性(IL1βr(S)=0.867；p
Objective-Digital image analysis (DIA) offers the opportunity to quantify the stained area and staining intensity when synovial tissue (ST) is investigated by immunohistochemical analysis. This study aimed at determining the sensitivity of DIA compared with semiquantitative analysis (SQA).Methods-Paired ST samples were obtained from the knee joint of 10 patients with rheumatoid arthritis (RA) with active disease and after follow up when complete clinical remission was achieved. ST samples of 10 subjects with noninflammatory knee pain served as controls. Immunohistochemistry with antibodies against interleukin 1 beta (IL1 beta) and vascular cell adhesion molecule 1 (VCAM-1) was applied using two staining proto cols with 3-amino-9-ethylcarbazole (AEC) or p-diethylaminobenzaldehyde (DAB) as dye. All sections were analysed semiquantitatively (0-4) and DIA of up to a maximum of 60 high power fields (HPF). The average integrated optical density was calculated as the product of the stained area (corrected for total tissue area) and the optical density.Results-Both SQA and DIA enabled the assessment of differences in IL1 beta and VCAM-1 expression between ST from active PA, RA in remission, and controls. SQA and DIA showed excellent correlations (IL1 beta r(s)=0.867; p