Immobilized E-cadherin model can enhance cell attachment and differentiation of primary hepatocytes but not proliferation
Immobilized E-cadherin model can enhance cell attachment and differentiation of primary hepatocytes but not proliferation
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DOI：
10.1023/a:1020905532227
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发表时间：
2002-11-01
影响因子：
2.7
通讯作者：
Akaike, T
中科院分区：
文献类型：
--
作者：
Nagaoka, M;Ise, H;Akaike, T
E-钙粘蛋白是调节细胞功能如细胞分化和增殖的细胞间粘附分子。为了阐明E-cadherin介导的粘附诱导分化的潜力，我们通过与免疫球蛋白G（IgG）Fc区融合构建了可吸附的重组E-cadherin分子（E-cad-Fc）。肝细胞可以通过E-钙粘蛋白的嗜同性相互作用粘附到融合蛋白包被的表面，并显示出分化的表型，如低DNA合成活性和色氨酸加氧酶表达的维持，类似于被称为高度分化的组织样细胞聚集的球状体形成的肝细胞。这些结果表明，E-cadherin是维持原代肝细胞分化的关键分子。
E-Cadherin is an intercellular adhesion molecule that regulates cell functions such as differentiation and proliferation of cells. To clarify the potential of E-cadherin-mediated adhesion to induce differentiation, we constructed an adsorbable recombinant E-cadherin molecule by fusing with an immunoglobulin G (IgG) Fc region (E-cad-Fc). Hepatocytes could adhere to the fusion protein-coated surface by a homophilic interaction of E-cadherins and showed differentiated phenotypes such as low DNA synthesizing activity and maintenance of tryptophan oxygenase expression, similar to those of spheroid-formed hepatocytes that are known as a highly differentiated tissue-like cell aggregation. These results suggest that E-cadherin is a key molecule for maintaining differentiation of primary hepatocytes.