DISTRIBUTION OF FOS-LIKE IMMUNOREACTIVITY IN THE CAUDAL BRAIN-STEM OF THE RAT FOLLOWING NOXIOUS CHEMICAL-STIMULATION OF THE TEMPOROMANDIBULAR-JOINT
DISTRIBUTION OF FOS-LIKE IMMUNOREACTIVITY IN THE CAUDAL BRAIN-STEM OF THE RAT FOLLOWING NOXIOUS CHEMICAL-STIMULATION OF THE TEMPOROMANDIBULAR-JOINT
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DOI：
10.1002/cne.903560311
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发表时间：
1995-06-05
影响因子：
2.5
通讯作者：
BEREITER, DA
中科院分区：
文献类型：
--
作者：
HATHAWAY, CB;HU, JW;BEREITER, DA
原癌基因c-fos的中枢表达被用来检测从大鼠颞下颌关节(TMJ)接受伤害性感觉输入的区域。单侧TMJ区注射小纤维特异性刺激剂/炎性刺激性芥子油2小时后，在尾侧脑干可见Fos样免疫反应(Fos-LI)。对照组动物将芥子油注入咬肌上覆的皮下筋膜，或将矿物油载体注入TMJ区域。在所有组中，Fos-LI在同侧三叉神经脊束核和颈髓背角，在双侧孤束核和延髓腹外侧区持续可见。Fos-LI在同侧三叉神经旁核的表达变化较大。在三叉神经感觉复合体内，Fos-LI主要分布于尾侧亚核和邻近OBEX水平的内侧亚核尾侧。在尾侧亚核和颈髓背角，约12%的Fos-LI细胞位于板层III-VI。与TMJ芥子油注射相比，矿物油注射在所有吻尾水平产生的Fos-LI较少，而皮下注射芥子油在尾侧亚核产生的Fos-LI较少，而在颈背角产生的Fos-LI与TMJ芥子油注射相似。这两种注射均未在TMJ尾侧亚核产生明显的同侧反应，表明TMJ芥子油注射后该区域的Fos-LI可能完全归因于TMJ深层区域的小纤维刺激。Fos-LI在尾侧脑干的广泛吻尾分布反映了TMJ反应伤害性神经元的分布，这可能是TMJ区疼痛传播和转介的基础。(C)1995年Wiley-Liss。Inc.
Central expression of the protooncogene c-fos was used to examine areas receiving noxious sensory input from the rat temporomandibular joint (TMJ). Fos-like immunoreactivity (Fos-LI) in the caudal brainstem was visualized 2 hours after unilateral injection of the small-fiber-specific excitant/inflammatory irritant mustard oil into the TMJ region. Control animals received injection of either mustard oil into the subcutaneous fascia overlying the masseter muscle or mineral oil vehicle into the TMJ region. In all groups, Fos-LI was consistently observed ipsilaterally in the spinal trigeminal nucleus and cervical dorsal horn and, bilaterally, in the nucleus of the solitary tract and the ventrolateral medulla. The expression of Fos-LI ipsilaterally in the paratrigeminal nucleus was variable. Within the trigeminal sensory complex, Fos-LI was restricted to subnucleus caudalis and the caudal portions of subnucleus interpolaris near the level of the obex. Approximately 12% of Fos-LI cells in subnucleus caudalis and in the cervical dorsal horn were found in laminae III-VI. Compared to TMJ mustard oil injection, mineral oil injection produced less Fos-LI at all rostrocaudal levels, whereas subcutaneous mustard oil injection produced less Fos-LI in caudal subnucleus caudalis but similar amounts in the cervical dorsal horn. Neither of these injections yielded significant ipsilateral responses in subnucleus caudalis, indicating that Fos-LI in this region following TMJ mustard oil injection could be ascribed solely to small-fiber stimulation in the deep TMJ region. The wide rostrocaudal distribution of Fos-LI within the caudal brainstem reflects the distribution of TMJ-responsive nociceptive neurons that may underlie the spread and referral of pain from the TMJ region. (C) 1995 Wiley-Liss. Inc.