COMPATIBILITY BETWEEN ENHANCERS AND PROMOTERS DETERMINES THE TRANSCRIPTIONAL SPECIFICITY OF GOOSEBERRY AND GOOSEBERRY NEURO IN THE DROSOPHILA EMBRYO
COMPATIBILITY BETWEEN ENHANCERS AND PROMOTERS DETERMINES THE TRANSCRIPTIONAL SPECIFICITY OF GOOSEBERRY AND GOOSEBERRY NEURO IN THE DROSOPHILA EMBRYO
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DOI：
10.1002/j.1460-2075.1994.tb06274.x
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发表时间：
1994-01-15
期刊：
影响因子：
11.4
通讯作者：
NOLL, M
中科院分区：
文献类型：
--
作者：
LI, XL;NOLL, M
果蝇的两个基因gooseberry（gsb）和gooseberry neuro（gsbn）是紧密并列的，并且转录不同。虽然gsb是一个节段极性基因，主要在表皮中表达，但gsbn在中枢神经系统中表达。一个有趣的问题是它们的转录特异性是如何产生的。在这里，我们表明，不同的非重叠增强子或上游控制元件驱动gsb和gsbn的特异性表达。这些增强子对其基因的特异性通过它们不能与另一基因的异源启动子组合激活转录来实现。因此，这些结果表明，增强子和其同源启动子之间的相容性是确保转录特异性的机制。
The two Drosophila genes gooseberry (gsb) and gooseberry neuro (gsbn) are closely apposed and divergently transcribed. While gsb is a segment-polarity gene and mainly expressed in the epidermis, gsbn is expressed in the central nervous system. An intriguing question is how their transcriptional specificity arises. Here we show that different non-overlapping enhancer or upstream control elements drive the specific expression of gsb and gsbn. Specificity of these enhancers for their genes is achieved by their inability to activate transcription in combination with the heterologous promoter of the other gene. These results therefore suggest that compatibility between the enhancer and its cognate promoter is a mechanism ensuring transcriptional specificity.