Molecular Assembly of Zinc Chlorophyll Derivatives by Using Recombin ant Light-Harvesting Polypeptides with His-tag and Immobilization on a Gold Electrode
Molecular Assembly of Zinc Chlorophyll Derivatives by Using Recombin ant Light-Harvesting Polypeptides with His-tag and Immobilization on a Gold Electrode
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利用带组氨酸标签的重组蚂蚁光捕获多肽分子组装并固定在金电极上的叶绿素锌衍生物
DOI：
10.1021/la400059h
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发表时间：
2013
期刊：
影响因子：
3.9
通讯作者：
Hideki Hashimoto and Mamoru Nango
中科院分区：
文献类型：
--
作者：
Shunsuke Sakai;Ibmoyasu Noji;Masaharu Kondo;Toshihisa Mizuno;Takehisa Dewa;Tsuyoshi Ochiai;Hisanori Yamakawa;Shigeru Itoh;Hideki Hashimoto and Mamoru Nango
LH 1-α和LH 1-β多肽与细菌叶绿素一起构成紫色光合细菌的捕光1（LH 1）复合物，沿着它们对各种卟啉类似物都具有独特的结合特性。在本文中，我们使用卟啉类似物，锌-二氢卟酚和锌-二氢卟酚二聚体，并检查它们与LH 1-α变体的结合行为，该变体在C-末端具有His-标签（MBP-rubα-YH）。Zn-Chlorin和Zn-Chlorin二聚体可与MBP-rub α-YH结合形成亚基型组装体，类似于天然LH 1复合物。这些配合物可以固定在镍-氨三乙酸修饰的Au电极上，并通过光照射成功地观察到阴极光电流。由于该络合物中的锌-二氢卟酚太远而不能从电极直接转移电子，因此暗示了多肽骨架对有效电子转移的贡献。这些发现不仅显示了LH 1-α多肽的有趣性质，而且为利用这些肽材料构建人工光合作用系统提供了线索。
LH1-α and -β polypeptides, which make up the light-harvesting 1 (LH1) complex of purple photosynthetic bacteria, along with bacteriochlorophylls, have unique binding properties even for various porphyrin analogs. Herein, we used the porphyrin analogs, Zn-Chlorin and the Zn-Chlorin dimer, and examined their binding behaviors to the LH1-α variant, which has a His-tag at the C-terminus (MBP-rubα-YH). Zn-Chlorin and the Zn-Chlorin dimer could bind to MBP-rubα-YH and form a subunit-type assembly, similar to that from the native LH1 complex. These complexes could be immobilized onto Ni-nitrilotriacetic acid-modified Au electrodes, and the cathodic photocurrent was successfully observed by photoirradiation. Since Zn-Chlorins in this complex are too far for direct electron transfer from the electrode, a contribution of polypeptide backbone for efficient electron transfer was implied. These findings not only show interesting properties of LH1-α polypeptides but also suggest a clue to construct artificial photosynthesis systems using these peptide materials.