Temporal and spatial expression of Drosophila Neurexin during the life cycle visualized using a DNRX-Gal4/UAS-reporter
Temporal and spatial expression of Drosophila Neurexin during the life cycle visualized using a DNRX-Gal4/UAS-reporter
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DOI：
10.1007/s11427-015-4946-9
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发表时间：
2015-10
期刊：
影响因子：
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通讯作者：
Mingkuan Sun;Xiankun Zeng;Wei Xie
中科院分区：
文献类型：
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作者：
Mingkuan Sun;Xiankun Zeng;Wei Xie
果蝇eurexin （Drosophilaneurexin， DNRX）在果蝇体内正常的结构发育和突触功能中起关键作用。然而，DNRX的时空表达模式尚不清楚。在这项研究中，我们产生了一种新的果蝇转基因菌株，称为DNRX- gal4转基因系，其特征与内源性DNRX表达模式一致。通过使用DNRX- gal4启动子驱动GFP报告基因（核定位和膜定位GFP）的表达来检测DNRX的表达。我们发现DNRX在胚胎、幼虫和成虫的中枢和运动神经元中优先表达，而在胶质细胞中不表达。DNRX在三龄幼虫神经肌肉连接（NMJs）突触前和突触后表达。报告基因在成虫的唾液腺、内脏、翅膀和腿中均有表达。在成人大脑中，包括蘑菇体（mb）、触角叶（AL）和视叶神经元在内的几个大脑区域都观察到报告基因的表达，抗体染色结果与内源性DNRX的表达一致。有趣的是，DNRX也在时钟神经元中表达。同时，我们发现DNRX在MBs中的表达是嗅觉学习和记忆所必需的。
Drosophilaneurexin (DNRX) plays a critical role in proper architecture development and synaptic functionin vivo. However, the temporal and spatial expression pattern of DNRX still remains unclear. For this study, we generated a novelDrosophilatransgenic strain termed the DNRX-Gal4 transgenic line, with characteristic features in agreement with the endogenous DNRX expression pattern. DNRX expression was examined by driving the expression of a GFP reporter (nuclear-localized and membrane- localized GFP) using the DNRX-Gal4 promoter. We found that DNRX was expressed preferentially in central and motor neurons in embryos, larvae and adults, but not in glial cells. DNRX was expressed in pre- and post-synaptic areas in third instar larvae neuromuscular junctions (NMJs). Reporter expression was also observed in the salivary glands, guts, wings and legs of adult flies. In the adult brain, reporter expression was observed throughout several brain regions, including the mushroom body (MBs), antennal lobe (AL) and optic lobe neurons, which is consistent with endogenous DNRX expression via antibody staining. Interestingly, DNRX was also expressed in clock neurons. Meanwhile, we found that DNRX expression in the MBs was required for olfactory learning and memory.