NEURON-SPECIFIC ENOLASE IN RELATION TO DIFFERENTIATION IN HUMAN NEURO-BLASTOMA
NEURON-SPECIFIC ENOLASE IN RELATION TO DIFFERENTIATION IN HUMAN NEURO-BLASTOMA
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DOI：
10.1016/0006-8993(81)91117-3
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
GROTTE, G
中科院分区：
文献类型：
--
作者：
ODELSTAD, L;PAHLMAN, S;GROTTE, G
用离子交换层析法对人神经母细胞瘤、神经节细胞瘤、神经节细胞瘤及培养的神经母细胞瘤细胞的活检标本进行了神经元特异性烯醇化酶(NSE)和非神经元烯醇化酶(NNE)两种形态的检测。在氯化钠的存在下，烯醇酶的活性受到抑制，但在用于层析步骤的氯化钾中保持活性。神经母细胞瘤组织中NSE的相对水平低于分化程度较高的神经节细胞瘤和神经节细胞瘤。体外培养的人神经母细胞瘤细胞系SK-N-SH、SH-SY5Y、SK-N-MC和IMR-32的NSE相对含量明显低于神经母细胞瘤活检组织。培养的细胞经神经生长因子或二丁酰cAMP处理后，部分细胞出现形态分化，NSE水平升高。NSE可作为判断神经母细胞瘤分化程度的指标。
The presence of 2 forms of enolase, neuron-specific enolase (NSE) and non-neuronal enolase (NNE), were examined in biopsy material of human neuroblastoma, ganglioneuroblastoma, ganglioneuroma and cultured neuroblastoma cells, after separation with ion exchange chromatography. The enolase activities were inhibited in the presence of NaCl but remained active in KCl, which were used in the chromatographic step. The relative NSE levels in the neuroblastoma tissues were lower than in the histopathologically more differentiated forms of the tumor, i.e., ganglioneuroblastoma and ganglioneuroma. The human neuroblastoma in vitro cell lines SK-N-SH, SH-SY5Y, SK-N-MC and IMR-32 contained considerably lower relative levels of NSE compared to the levels in the neuroblastoma biopsies. After treatment of the cultured cells with nerve growth factor or dibutyryl-cAMP some cells showed morphological differentiation and concomitantly an increase in the NSE levels. NSE might be useful as a marker for differentiation in human neuroblastoma.