Cortical fosGFP Expression Reveals Broad Receptive Field Excitatory Neurons Targeted by POm
Cortical fosGFP Expression Reveals Broad Receptive Field Excitatory Neurons Targeted by POm
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DOI：
10.1016/j.neuron.2014.10.014
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发表时间：
2014-12-03
期刊：
影响因子：
16.2
通讯作者：
Poulet, James F. A.
中科院分区：
文献类型：
--
作者：
Jouhanneau, Jean-Sebastien;Ferrarese, Leiron;Poulet, James F. A.
邻近的皮质兴奋性神经元对感觉刺激的反应表现出相当大的异质性。我们假设幼年(18至27岁)小鼠须体感皮层中的一组第二层兴奋性神经元，以活性依赖的fosGFP报告基因的表达为特征，将优先被胡须刺激激活。事实上，双光子靶向双全细胞记录显示，主要胡须刺激在表达fosGFP的神经元和fosGFP(-)神经元中引起类似的幅度突触反应。相反，FosGFP(+)神经元对周围胡须刺激表现出更短的潜伏期和更大的亚阈值反应幅度。利用光遗传刺激，我们确定这些神经元是来自后内侧核(POM)的轴突的靶点，后内侧核是丘脑旁核的一个与广泛的感受野和广泛的皮质投射相关的核。我们的结论是，fosGFP的表达区分了单须和多须感受区第二层锥体神经元。
Neighboring cortical excitatory neurons show considerable heterogeneity in their responses to sensory stimulation. We hypothesized that a subset of layer 2 excitatory neurons in the juvenile (P18 to 27) mouse whisker somatosensory cortex, distinguished by expression of the activity-dependent fosGFP reporter gene, would be preferentially activated by whisker stimulation. In fact, two-photon targeted, dual whole-cell recordings showed that principal whisker stimulation elicits similar amplitude synaptic responses in fosGFP-expressing and fosGFP(-) neurons. FosGFP(+) neurons instead displayed shorter latency and larger amplitude subthreshold responses to surround whisker stimulation. Using optogenetic stimulation, we determined that these neurons are targeted by axons from the posteromedial nucleus (POm), a paralemniscal thalamic nucleus associated with broad receptive fields and widespread cortical projections. We conclude that fosGFP expression discriminates between single-and multi-whisker receptive field layer 2 pyramidal neurons.