Inducing Secondary Metabolite Production by the Endophytic Fungus Fusarium tricinctum through Coculture with Bacillus subtilis
Inducing Secondary Metabolite Production by the Endophytic Fungus Fusarium tricinctum through Coculture with Bacillus subtilis
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DOI：
10.1021/np400589h
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发表时间：
2013-11-01
影响因子：
5.1
通讯作者：
Prolcsch, Peter
中科院分区：
文献类型：
--
作者：
Ola, Antonius R. B.;Thomy, Dhana;Prolcsch, Peter
在固体水稻培养基上共培养真菌内生真菌三环镰孢菌与细菌枯草芽孢杆菌168 trpC 2导致组成型存在的次级代谢产物的积累增加高达78倍，所述次级代谢产物包括侧吡酮（5）、恩镰孢菌素类型的环状缩酚肽（6-8）和脂肽镰孢菌素A（9）。此外，四种化合物（1-4），包括（-）-柠檬酸异香豆素（2）以及三种新的天然产物（1，3和4）不存在于离散的真菌和细菌对照中，仅在共培养物中检测到。通过1D和2D NMR和HRMS数据分析，新化合物被鉴定为macrocarpon C（1），2-（羧甲基氨基）苯甲酸（3）和（-）-citreoisocoumarinol（4）。恩尼亚汀B1（7）和A1（8）的产量特别高，抑制共培养B的生长。枯草杆菌菌株的最小抑菌浓度（MIC）分别为16和8 μ g/mL，并且还对金黄色葡萄球菌、肺炎链球菌和粪肠球菌具有活性，MIC值在2-8 μ g/mL范围内。此外，在F.三环草对B具有较好的抗菌活性。枯草芽孢杆菌S.金黄色葡萄球菌、pneumoniae和E. faecalis，MIC值范围为2 - 8 μ g/mL。所有活性化合物对多重耐药临床分离的沙门氏菌同样有效。金黄色葡萄球菌和同一种属的敏感参考菌株。
Coculturing the fungal endophyte Fusarium tricinctum with the bacterium Bacillus subtilis 168 trpC2 on solid rice medium resulted in an up to 78-fold increase in the accumulation in constitutively present secondary metabolites that included lateropyrone (5), cyclic depsipeptides of the enniatin type (6-8), and the lipopeptide fusaristatin A (9). In addition, four compounds (1-4) including (-)-citreoisocoumarin (2) as well as three new natural products (1, 3, and 4) were not present in discrete fungal and bacterial controls and only detected in the cocultures. The new compounds were identified as macrocarpon C (1), 2-(carboxymethylamino)benzoic acid (3), and (-)-citreoisocoumarinol (4) by analysis of the 1D and 2D NMR and HRMS data. Enniatins B1 (7) and A1 (8), whose production was particularly enhanced, inhibited the growth of the cocultivated B. subtilis strain with minimal inhibitory concentrations (MICs) of 16 and 8 mu g/mL, respectively, and were also active against Staphylococcus aureus, Streptococcus pneumoniae, and Enterococcus faecalis with MIC values in the range 2-8 mu g/mL. In addition, lateropyrone (5), which was constitutively present in F. tricinctum, displayed good antibacterial activity against B. subtilis, S. aureus, S. pneumoniae, and E. faecalis, with MIC values ranging from 2 to 8 mu g/mL. All active compounds were equally effective against a multiresistant clinical isolate of S. aureus and a susceptible reference strain of the same species.