Tissue distribution of 35S-labelled perfluorooctane sulfonate in adult mice after oral exposure to a low environmentally relevant dose or a high experimental dose
Tissue distribution of 35S-labelled perfluorooctane sulfonate in adult mice after oral exposure to a low environmentally relevant dose or a high experimental dose
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DOI：
10.1016/j.tox.2011.03.014
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发表时间：
2011-06-18
期刊：
影响因子：
4.5
通讯作者：
Nobel, Stefan
中科院分区：
文献类型：
--
作者：
Bogdanska, Jasna;Borg, Daniel;Nobel, Stefan
在大多数动物物种（包括普通人群）中检测到的广泛存在的环境污染物全氟辛烷磺酸（PFOS）对实验动物产生了多种影响，例如，肝毒性、免疫毒性和发育毒性。然而，关于全氟辛烷磺酸在哺乳动物体内组织分布的详细信息很少，特别是缺乏与环境相关的接触水平的可用信息，限制了我们对哺乳动物（包括人类）可能受到影响的了解。因此，我们确定了该化合物在小鼠体内的组织分布特征，小鼠是研究全氟辛烷磺酸毒性的重要实验动物。成年雄性C57/BL 6小鼠在饮食中暴露于环境相关的（0.031 mg/kg/天）或750倍以上的实验相关剂量（23毫克/千克/天）的S-35-全氟辛烷磺酸，大部分给药的放射性在肝脏、骨骼在肝脏、肺、血液、肾脏和骨骼（骨髓）中检测到的浓度最高。在高日剂量接触后，全氟辛烷磺酸的分布情况与低日剂量接触时不同，这表明随着剂量的增加，全氟辛烷磺酸的分布从血液转移到组织。闪烁计数（对组织中存在的血液进行了校正）和全身放射自显影术均显示，在所有19种检查的组织中均存在全氟辛烷磺酸，并确定胸腺是全氟辛烷磺酸的新定位部位，骨骼（骨髓）、皮肤和肌肉是全氟辛烷磺酸的重要身体部位。这些研究结果表明，全氟辛烷磺酸以剂量依赖的方式离开血液并进入大多数组织。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
The widespread environmental pollutant perfluorooctane sulfonate (PFOS), detected in most animal species including the general human population, exerts several effects on experimental animals, e.g., hepatotoxicity, immunotoxicity and developmental toxicity. However, detailed information on the tissue distribution of PFOS in mammals is scarce and, in particular, the lack of available information regarding environmentally relevant exposure levels limits our understanding of how mammals (including humans) may be affected. Accordingly, we characterized the tissue distribution of this compound in mice, an important experimental animal for studying PFOS toxicity. Following dietary exposure of adult male C57/BL6 mice for 1-5 days to an environmentally relevant (0.031 mg/kg/day) or a 750-fold higher experimentally relevant dose (23 mg/kg/day) of S-35-PFOS, most of the radioactivity administered was recovered in liver, bone (bone marrow), blood, skin and muscle, with the highest levels detected in liver, lung, blood, kidney and bone (bone marrow). Following high daily dose exposure, PFOS exhibited a different distribution profile than with low daily dose exposure, which indicated a shift in distribution from the blood to the tissues with increasing dose. Both scintillation counting (with correction for the blood present in the tissues) and whole-body autoradiography revealed the presence of PFOS in all 19 tissues examined, with identification of thymus as a novel site for localization for PFOS and bone (bone marrow), skin and muscle as significant body compartments for PFOS. These findings demonstrate that PFOS leaves the bloodstream and enters most tissues in a dose-dependent manner. (C) 2011 Elsevier Ireland Ltd. All rights reserved.