ELECTROPHYSIOLOGY OF GLOBUS-PALLIDUS NEURONS IN-VITRO
ELECTROPHYSIOLOGY OF GLOBUS-PALLIDUS NEURONS IN-VITRO
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DOI：
10.1152/jn.1994.72.3.1127
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发表时间：
1994-09-01
影响因子：
2.5
通讯作者：
LLINAS, R
中科院分区：
文献类型：
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作者：
NAMBU, A;LLINAS, R
1. 我们利用成年豚鼠的脑切片研究了苍白球神经元细胞内的电特性。根据其固有的电生理特性，将神经元分为三种类型。I型神经元（59%）在静息膜水平（-65 +/- 10 mV，平均+/- SD）沉默，并对去极化电流注入产生强调节的脉冲。钙依赖的低频（1-8 Hz）膜振荡通常由膜去极化（-53 +/- 8 mV）引起。低阈值钙电导和A电流也被确定。该神经元型的平均输入电阻为70 +/- 22 M ω .3。II型神经元（37%）在静息膜水平自发放电（-59 +/- 9 mV）。他们的重复放电（小于或等于200赫兹）对注入电流的幅度非常敏感，并表现出弱调节。钠依赖的高频（20-100 Hz）阈下膜振荡通常由膜去极化引起。这类神经元表现出低阈值的钙峰值，并且在三种神经元类型中具有最高的输入电阻（134 +/- 62 M ω）。III型神经元（4%）在静息膜水平（-73 +/- 5 mV）没有自发放电。动作电位持续时间长（2.3 +/- 0.6 ms）。去极化电流注入引起的重复放电表现为弱调节或无调节。该神经元类型具有a电流，并且显示出三种神经元类型中最低的输入电阻（52 +/- 35 M ω）。尾核刺激可诱发I型和II型神经元的突触后抑制电位（IPSPs）。在II型神经元中，ipsp之后通常是反弹放电。兴奋性突触后电位和反节律反应也在一些I型和II型神经元中引起。估计纹状体投射的传导速度为
1. We investigated the electrical properties of globus pallidus neurons intracellularly using brain slices from adult guinea pigs. Three types of neurons were identified according to their intrinsic electrophysiological properties.2. Type I neurons (59%) were silent at the resting membrane level (-65 +/- 10 mV, mean +/- SD) and generated a burst of spikes, with strong accommodation, to depolarizing current injection. Calcium-dependent low-frequency (1-8 Hz) membrane oscillations were often elicited by membrane depolarization (-53 +/- 8 mV). A low-threshold calcium conductance and an A-current were also identified. The mean input resistance of this neuronal type was 70 +/- 22 M Omega.3. Type II neurons (37%) fired spontaneously at the resting membrane level (-59 +/- 9 mV). Their repetitive firing (less than or equal to 200 Hz) was very sensitive to the amplitude of injected current and showed weak accommodation. Sodium-dependent high-frequency (20-100 Hz) subthreshold membrane oscillations were often elicited by membrane depolarization. This neuronal type demonstrated a low-threshold calcium spike and had the highest input resistance (134 +/- 62 M Omega) of the three neuron types.4. Type III neurons (4%) did not fire spontaneously at the resting membrane level (-73 +/- 5 mV). Their action potentials were characterized by a long duration (2.3 +/- 0.6 ms). Repetitive firing elicited by depolarizing current injection showed weak or no accommodation. This neuronal type had an A-current and showed the lowest input resistance (52 +/- 35 M Omega) of the three neuron types.5. Stimulation of the caudoputamen evoked inhibitory postsynaptic potentials (IPSPs) in Type I and II neurons. In Type II neurons the IPSPs were usually followed by rebound firing. Excitatory postsynaptic potentials and antidromic responses were also elicited in some Type I and II neurons. The estimated conduction velocity of the striopallidal projection was