Increased frequencies of both chromosome abnormalities and SCEs in two sheep flocks exposed to high dioxin levels during pasturage
Increased frequencies of both chromosome abnormalities and SCEs in two sheep flocks exposed to high dioxin levels during pasturage
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DOI：
10.1093/mutage/gei076
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发表时间：
2006-01-01
期刊：
影响因子：
2.7
通讯作者：
Iannuzzi, L
中科院分区：
文献类型：
--
作者：
Perucatti, A;Di Meo, GP;Iannuzzi, L
在过去四年中，那不勒斯省和卡塞塔省（意大利南部）的几个牲畜养殖场（羊、牛和水牛）无法出售其牛奶和其他乳制品，原因是牛奶质量中二恶英（17种不同类型）的含量超过了允许的值[3毫克/克脂肪，世卫组织2,3,7,8-四氯二苯并对二恶英（TCDD或二恶英）当量]。虽然一些农场，特别是那些二恶英含量相对较低的农场，已经设法通过改变饮食将牛奶中的二恶英含量降低到允许的阈值以下，但许多农场，特别是绵羊农场，并没有这样做。事实上，到目前为止，大约有1.2万头牛、水牛和羊被宰杀。在本研究中，对那不勒斯省（阿塞拉市）饲养的两个羊群中的34只和42只羊进行了细胞遗传学调查，并与距离暴露区80公里外饲养的20只羊（对照）进行了比较。这些羊群的乳汁中二恶英含量较高（脂肪含量分别为50.65和39.51 pg/g）。与对照组相比，两组奶牛的染色体异常（间隙、染色体断裂和染色单体断裂）和姐妹染色单体交换（sce）均增加（分别是对照组的17倍和8倍），且差异极显著（P < 0.001）。暴露动物的非整倍体细胞频率（16.4%和17.8%）与对照组（17.9%）比较，差异无统计学意义。此外，与对照组相比，两个农场中的一个记录了高水平的死亡率和异常胎儿。
During the past four years several livestock farms (sheep, cattle and river buffalo) in the provinces of Naples and Caserta (southern Italy) have been unable to sell their milk and other dairy products due to the levels of dioxins (17 different types) present in the milk mass exceeding the value permitted [3 pg/g of fat, as human WHO 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD or dioxin) equivalent]. While some farms, especially those showing relatively low levels of dioxins, have managed to reduce the dioxins in the milk below the permitted threshold by changing the diet, many, especially sheep farms, have failed to do so. Indeed, about 12 000 head of cattle, river buffaloes and sheep have so far been culled. In the present study, 34 and 42 sheep from two herds raised in the province of Naples (Acerra municipality) and where high levels of dioxins (50.65 and 39.51 pg/g of fat, respectively) were found in the milk mass, were cytogenetically investigated and compared with 20 sheep (control) raised 80 km away from the exposed area. Increases of both chromosome abnormalities (gap, chromosome and chromatid breaks) (17 and 8 times higher in the two exposed herds, respectively) and sister chromatid exchanges (SCEs) were found in both herds when compared with the control, and the differences were highly significant (P < 0.001). No statistical differences were found when comparing the frequencies of aneuploid cells of exposed animals (16.4 and 17.8%) and control (17.9%). Furthermore, high levels of mortality and abnormal foetuses were recorded in one of the two farms when compared with the control.