Technoeconomic evaluation of bio-based styrene production by engineered Escherichia coli
Technoeconomic evaluation of bio-based styrene production by engineered Escherichia coli
复制标题
DOI：
10.1007/s10295-014-1469-5
复制
发表时间：
2014-08-01
影响因子：
3.4
通讯作者：
Nielsen, David R.
中科院分区：
文献类型：
--
作者：
Claypool, Joshua T.;Raman, D. Raj;Nielsen, David R.
苯乙烯是用于聚合物和树脂的重要商品化学品，并且通常由石油化工原料苯和乙烯生产。苯乙烯最近首次使用工程大肠杆菌生物合成生产，这种生物基路线可能代表石油衍生苯乙烯的低能耗和可再生替代品。然而，这种方法的经济性尚未得到研究。利用早期技术经济评价工具，对以C-6-糖为原料生产生物基苯乙烯进行了初步的经济分析。由于苯乙烯有限的水溶性，假定所得发酵液将自发地形成两个不混溶的液相，其随后可被倾析。假设目前的C-6糖价格和工业上可实现的生物动力学参数值（例如，产品产率，比生长率），商业规模的生物基苯乙烯的最低估计售价（MESP）为1.90美元/千克（-1），这在当前苯乙烯价格范围内。蒙特卡洛分析显示，MESP的标准差可能很大（0.45美元/千克），而敏感性分析显示，原料价格和总产量是MESP的主要驱动因素。
Styrene is an important commodity chemical used in polymers and resins, and is typically produced from the petrochemical feedstocks benzene and ethylene. Styrene has recently been produced biosynthetically for the first time using engineered Escherichia coli, and this bio-based route may represent a lower energy and renewable alternative to petroleum-derived styrene. However, the economics of such an approach has not yet been investigated. Using an early-stage technoeconomic evaluation tool, a preliminary economic analysis of bio-based styrene from C-6-sugar feedstock has been conducted. Owing to styrene's limited water solubility, it was assumed that the resulting fermentation broth would spontaneously form two immiscible liquid phases that could subsequently be decanted. Assuming current C-6 sugar prices and industrially achievable biokinetic parameter values (e.g., product yield, specific growth rate), commercial-scale bio-based styrene has a minimum estimated selling price (MESP) of 1.90 USD kg(-1) which is in the range of current styrene prices. A Monte Carlo analysis revealed a potentially large (0.45 USD kg(-1)) standard deviation in the MESP, while a sensitivity analysis showed feedstock price and overall yield as primary drivers of MESP.