Low Nitrogen Stress Stimulated Nitrate Uptake Rate Modulated by Auxin in Brassica napus L.
Low Nitrogen Stress Stimulated Nitrate Uptake Rate Modulated by Auxin in Brassica napus L.
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DOI：
10.1007/s42729-022-00904-x
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发表时间：
2022-06
影响因子：
3.9
通讯作者：
Lan Yang;Shang Luo;Zhimin Wu;Xiangmin Rong;Yongliang Han
中科院分区：
文献类型：
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作者：
Lan Yang;Shang Luo;Zhimin Wu;Xiangmin Rong;Yongliang Han
为了探讨生长素调节甘蓝型油菜硝酸盐吸收速率的机制。在低氮胁迫下。试验分四个处理：(1)15M，植株生长在正常氮(N)溶液中(N03−：15−/L)；(2)1M，植株生长在低N胁迫溶液(NO3 + ：1−/L)中；(3)15MIAA，含有生长素的正常供氮溶液(IAA，10 + 8mol/L)；(4)1MIAA，生长在低N胁迫溶液中的植物，添加5μ/L N-1-萘基邻苯二甲酸(NPA，一种生长素极性运输的抑制剂)。测定生长素含量、NO3-−摄取率、BnNRT1.1表达、PM H+-ATPase活性、生长素合成和极地运输相关基因的表达。与15M相比，1M处理的N3IAA吸收速率、根系生长素含量、生长素合成数量和极性运输相关基因均显著增加。1M处理比15M处理增加了29.20%的根系−，70.80%来源于根系合成。低氮胁迫促进了生长素的原位合成和地上部对生长素的极地运输，提高了根系生长素含量，增强了−的表达和PM H+- + 酶活性，提高了−的表达和PM H+-ATPase活性，提高了N3- + 的吸收速率，从而提高了N3−的吸收速率。
In order to investigate the mechanisms of auxin regulation on the stimulation of nitrate uptake rate ofBrassica napusL. under low nitrogen stress. Four treatments were applied: (1) 15 M, plants grown in normal nitrogen (N) solution (NO3−: 15 mmol/L); (2) 1 M, plants grown in low N stress solution (NO3−: 1 mmol/L); (3) 15 M + IAA, the normal N supply solution contain auxin (IAA, 10−8mol/L); and (4) 1 M + NPA, plants grown in low N stress solution with 5 μmol/L N-1-naphthylphthalamic acid (NPA, an inhibitor of polar auxin transport) applied to the rootstocks. The contents of auxin, NO3−uptake rate, the expression of BnNRT1.1, PM H+-ATPase activity, and the genes expression involved in the auxin synthesis and polar transport were all assayed. The NO3−uptake rate, root auxin content, a number of auxin synthesis, and polar transport related genes in 1 M were significantly increased than that of 15 M. The root IAA increment in 1 M compared with 15 M, originated from root synthesis shared with 29.20% and polar transport from shoot shared with 70.80%. The expression of BnNRT1.1 and PM H+-ATPase activity were significantly promoted in 1 M compared with 15 M. In addition, the NO3−uptake rate in 15 M + IAA was significantly higher than that of 15 M, and the NO3−uptake rate in 1 M + NPA was significantly lower than that of 1 M. Because of low N stress promoted in situ synthesis of auxin in root and its polar transport from shoot, the root auxin content was increased, enhanced the expression of BnNRT1.1 and PM H+-ATPase activity, resulting in higher NO3−uptake rate.