Lactobacillus plantarum DK119 as a Probiotic Confers Protection against Influenza Virus by Modulating Innate Immunity
Lactobacillus plantarum DK119 as a Probiotic Confers Protection against Influenza Virus by Modulating Innate Immunity
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DOI：
10.1371/journal.pone.0075368
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发表时间：
2013-10-04
期刊：
影响因子：
3.7
通讯作者：
Kang, Sang-Moo
中科院分区：
文献类型：
--
作者：
Park, Min-Kyung;Vu Ngo;Kang, Sang-Moo
从发酵大白菜食品中分离得到植物乳杆菌DK119 （DK119）作为益生菌，研究其对流感病毒的抗病毒作用。在小鼠模型中，经鼻或口服给药DK119对随后的甲型流感病毒致命感染具有100%的保护作用，防止体重显著减轻，并降低肺病毒载量。观察DK119给药的抗病毒保护作用呈剂量依赖性和途径依赖性。用DK119治疗的小鼠在支气管肺泡灌洗液中显示出高水平的细胞因子IL-12和ifn - γ，并且在感染流感病毒后出现低程度的炎症。肺泡巨噬细胞在肺部的耗竭和支气管肺泡灌洗完全消除了dk119介导的保护作用。调节宿主树突状细胞和巨噬细胞的先天免疫以及细胞因子的产生模式可能是DK119对流感病毒感染表现出抗病毒作用的机制。这些结果表明，DK119可以作为一种有益的抗病毒益生菌微生物开发。
Lactobacillus plantarum DK119 (DK119) isolated from the fermented Korean cabbage food was used as a probiotic to determine its antiviral effects on influenza virus. DK119 intranasal or oral administration conferred 100% protection against subsequent lethal infection with influenza A viruses, prevented significant weight loss, and lowered lung viral loads in a mouse model. The antiviral protective efficacy was observed in a dose and route dependent manner of DK119 administration. Mice that were treated with DK119 showed high levels of cytokines IL-12 and IFN-gamma in bronchoalveolar lavage fluids, and a low degree of inflammation upon infection with influenza virus. Depletion of alveolar macrophage cells in lungs and bronchoalveolar lavages completely abrogated the DK119-mediated protection. Modulating host innate immunity of dendritic and macrophage cells, and cytokine production pattern appeared to be possible mechanisms by which DK119 exhibited antiviral effects on influenza virus infection. These results indicate that DK119 can be developed as a beneficial antiviral probiotic microorganism.