Mortality, temporal substrate and insulin responses to endotoxic shock in zero, ten and twenty-eight day old rats.
Mortality, temporal substrate and insulin responses to endotoxic shock in zero, ten and twenty-eight day old rats.
复制标题
零日龄、十日龄和二十八日龄大鼠的死亡率、时间底物和胰岛素对内毒素休克的反应。
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发表时间：
1991
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通讯作者：
R. M. Hurley
中科院分区：
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作者：
W. P. Zeller;M. Gotō;L. Witek;R. M. Hurley
新生儿败血症是一个严重的健康问题。然而，据我们所知，尚未对幼年动物的时间底物和胰岛素对内毒素的反应进行表征，以指导新生儿感染性休克的血糖调节研究。我们表征了发育中的大鼠对内毒素的时间反应。对0日龄、10日龄和28日龄大鼠腹膜内给予高致死剂量和低致死剂量的Sprague-Dawley大鼠腹膜内肠炎沙门氏菌内毒素。监测死亡率、暂时葡萄糖、乳酸、肝糖原和胰岛素。死亡率实验表明，10天龄的大鼠对内毒素的敏感度是28天龄大鼠的300倍。高死亡率组中零日龄和十日龄大鼠的血浆葡萄糖浓度增加了 120 分钟（分别为 102 +/- 4 毫克/分升、119 +/- 6 毫克/分升），28 日龄大鼠的血浆葡萄糖浓度增加了 60 分钟（223 +/- 12 毫克/分升）。血浆葡萄糖浓度下降至 52 +/- 3 毫克/分升。 10 日龄大鼠的 240 分钟和 28 天高死亡率组中的 180 分钟至 99 +/- 8 毫克/分升，零日龄大鼠的峰值乳酸水平为 2.8 +/- 0.2 毫摩尔/升，28 日龄大鼠的肝脏中的糖原迅速下降 120。在 0 日龄和 10 日龄大鼠中，血浆胰岛素浓度没有显着升高，在 28 日龄大鼠中，胰岛素浓度增加了 120 分钟，达到 52 +/- 17 微单位/毫升，表明高胰岛素血症。
Neonatal sepsis is a significant health problem. However, to our knowledge, the temporal substrates and insulin response to endotoxin have not been characterized in the young animal to guide the investigations of glucoregulation in septic shock in the newborn. We characterized the temporal response to endotoxin in the developing rat. Sprague-Dawley rats were given intraperitoneal Salmonella enteritidis endotoxin in high and low lethal doses to zero, ten and 28 day old rats. Mortality, temporal glucose, lactate, hepatic glycogen and insulin were monitored. Mortality experiments show the ten day old rat is 300 times as sensitive to endotoxin as the 28 day old rat. Plasma glucose concentration increased in the high mortality groups by 120 minutes in the zero and ten day old rats (102 +/- 4 milligrams per deciliter, 119 +/- 6 milligrams per deciliter, respectively, and by 60 minutes in the 28 day old rats (223 +/- 12 milligrams per deciliter). The plasma glucose level decreased to 52 +/- 3 milligrams per deciliter by 240 minutes in the ten day old and by 180 minutes to 99 +/- 8 milligrams per deciliter in the 28 day high mortality groups. Peak lactic acid levels in the high lethality groups were zero day 2.8 +/- 0.2 millimoles per liter in zero day old rats, 3.3 +/- 0.2 millimoles per liter in 28 day old rats. Glycogen in the liver decreased rapidly by 120 minutes in all age groups. Plasma insulin concentration did not elevate significantly in zero and ten day old rats. In the 28 day old rat, insulin concentration increased by 120 minutes to 52 +/- 17 microunits per milliliter. Insulin glucose ratios were also elevated in the 28 day old endotoxin treated rat, indicating hyperinsulinemia. Thus, temporal substrates and insulin responses to endotoxin differ with animal age.