In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species - A study of the production of extracellular metabolites by Trichoderma species
In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species - A study of the production of extracellular metabolites by Trichoderma species
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DOI：
10.1023/a:1006802423729
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发表时间：
1997-01-01
期刊：
影响因子：
5.5
通讯作者：
Berjak, P
中科院分区：
文献类型：
--
作者：
Calistru, C;McLean, M;Berjak, P
绿色木霉和哈茨木霉的培养物滤液对串珠镰刀菌有抑制作用，并且在较小程度上抑制黄曲霉。然而，抑制程度取决于掺入培养基中的碳源或氮源。扫描电子显微镜显示，暴露于一些木霉培养物滤液后，会出现异常的子实体结构，而宏观上则观察到生长受限，并且在黄曲霉的情况下，观察到菌落颜色改变。根据倒置菌落培养的结果，木霉属的一些分离株似乎产生抑制性挥发性化合物。还证明了可能的抗生素的生产。木霉菌表现出的攻击行为（针对黄曲霉菌和串珠镰刀菌），部分可以通过这些真菌释放胞外酶来解释。绿色木霉的分离物表现出淀粉分解、果胶分解、蛋白水解和纤维素分解活性。根据目前的调查结果，木霉属 spp。是一些产生霉菌毒素的真菌的生物防治的潜在候选者，但对其风干种子内的渗透压耐受性存在一些疑问。
Culture filtrates of Trichoderma viride and Trichoderma harzianum were inhibitory of Fusarium moniliforme and, to a lesser extent, Aspergillus flavus. The degree of inhibition was, however, dependent on the carbon or nitrogen source incorporated into the medium. Scanning electron microscopy revealed the development of abnormal fruiting structures on exposure to some Trichoderma culture filtrate, while macroscopically, growth restriction and, in the case of A. flavus, altered colony colouration were observed. Based on the results of inverted colony culture, it would appear that some isolates of Trichoderma produce inhibitory volatile compounds. The production of possible antibiotics was also demonstrated. The aggressive behaviour (towards A. flavus and F. moniliforme) demonstrated by Trichoderma spp, may be partly explained by the liberation of extracellular enzymes by these fungi. An isolate of T. viride exhibited amylolytic, pectinolytic, proteolytic and cellulolytic activity. Based on the results of the present investigation, Trichoderma spp. are potential candidates for biocontrol of some mycotoxin-producing fungi, but there exists some doubt as to their osmotolerance within the air-dry seed.