In vitro and in vivo electron transfer to the triheme cytochrome subunit bound to the photosynthetic reaction center complex in the purple bacterium Rhodovulum sulfidophilum.
In vitro and in vivo electron transfer to the triheme cytochrome subunit bound to the photosynthetic reaction center complex in the purple bacterium Rhodovulum sulfidophilum.
复制标题
体外和体内电子转移到与紫色细菌嗜硫红酵母中光合反应中心复合体结合的三血红素细胞色素亚基。
DOI：
10.1016/s0005-2728(01)00177-3
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
K. Matsuura
中科院分区：
文献类型：
--
作者：
M. Yoshida;S. Masuda;K. Nagashima;A. Verméglio;K. Shimada;K. Matsuura
在其它紫色细菌中发现的四个基序中，与Rhodovulum sulfidophilum中的光合反应中心（RC）复合物结合的细胞色素亚基缺少一个血红素结合基序（CXXCH），导致RC-核心复合物（S.增田等人，J.Biol.Chem.274（1999）10795）。纯化了作为RC电子供体的细胞色素c2，并克隆了其基因并测序。细胞色素c2的氧化还原中点电位为Em=357 mV。在膜制剂存在下，观察到纯化的细胞色素c2的光氧化和再还原。在完整细胞中也观察到闪光诱导的光氧化和RC结合的细胞色素的再还原。尽管RC结合的细胞色素亚基的不寻常的性质，在Rdv的环状电子转移系统。嗜硫菌与其它紫色细菌的类似。
The cytochrome subunit bound to the photosynthetic reaction center (RC) complex in Rhodovulum sulfidophilum lacks one heme-binding motif (CXXCH) out of four motifs found in other purple bacteria resulting in the absence of the most distal heme from the RC-core complex (S. Masuda et al., J. Biol. Chem. 274 (1999) 10795). Cytochrome c2, which acts as the electron donor to the RC was purified, and its gene was cloned and sequenced. The redox midpoint potential of cytochrome c2was determined to be Em=357 mV. The photo-oxidation and re-reduction of purified cytochrome c2were observed in the presence of membrane preparations. Flash-induced photo-oxidation and re-reduction of the RC-bound cytochrome were also observed in intact cells. Despite the unusual nature of the RC-bound cytochrome subunit, the cyclic electron transfer system in Rdv. sulfidophilum was shown to be similar to those in other purple bacteria.
A.Osyczka 等人：“通过定点诱变绘制的与细菌光合反应中心结合的四血红素细胞色素亚基上可溶性细胞色素的相互作用位点”生物化学。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--