Somatostatin and nociceptin inhibit neurons in the central nucleus of amygdala that project to the periaqueductal grey
Somatostatin and nociceptin inhibit neurons in the central nucleus of amygdala that project to the periaqueductal grey
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DOI：
10.1016/j.neuropharm.2010.06.001
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发表时间：
2010-11-01
影响因子：
4.7
通讯作者：
Christie, MacDonald J.
中科院分区：
文献类型：
--
作者：
Chieng, Billy;Christie, MacDonald J.
杏仁中央核（CeA）在下行性抗伤害感受通路的调节中起重要作用。采用全细胞膜片钳技术，我们发现CeA神经元对内源性配体生长抑素（SST）和孤啡肽/孤啡肽（OFQ）的反应是通过增加K-电导来实现的。与选择性拮抗剂合用表明SST和OFQ分别作用于SSTR 2和ORL 1受体。考虑到记录的神经元的解剖定位，本研究表明，许多响应神经元位于内侧亚部的CeA和所有的CeA投射神经元中脑导水管周围灰质总是响应这些肽。随机选择的激动剂反应神经元在CeA主要分类生理低阈值尖峰神经元。SST，OFQ和，如前所述，阿片类药物的反应在CeA神经元的亚群的相似性表明收敛的作用，这些肽抑制从CeA到vIPAG的投射的活性，并可能类似的抗伤害作用，在这一途径。(C)2010爱思唯尔有限公司版权所有。
The central nucleus of amygdala (CeA) plays an important role in modulation of the descending antinociceptive pathways. Using whole-cell patch clamp recordings from brain slices, we found that CeA neurons responded to the endogenous ligands somatostatin (SST) and nociceptin/orphanin FQ (OFQ) via an increased K-conductance. Co-application with selective antagonists suggested that SST and OFQ act on SSTR2 and ORL1 receptors, respectively. Taking account of anatomical localisation of recorded neurons, the present study showed that many responsive neurons were located within the medial subdivision of CeA and all CeA projection neurons to the midbrain periaqueductal grey invariably responded to these peptides. Randomly selected agonist-responsive neurons in CeA predominantly classified physiologically as low-threshold spiking neurons. The similarity of SST, OFQ and, as previously reported, opioid responsiveness in a sub-population of CeA neurons suggests converging roles of these peptides to inhibit the activity of projections from CeA to vIPAG, and potentially similar antinociceptive actions in this pathway. (C) 2010 Elsevier Ltd. All rights reserved.