Encephalomyelitis of mice I. Characteristics and pathogenesis of the virus
Encephalomyelitis of mice I. Characteristics and pathogenesis of the virus
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DOI：
10.1084/jem.72.1.49
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发表时间：
1940-07-01
影响因子：
15.3
通讯作者：
Gard, S
中科院分区：
文献类型：
--
作者：
Theiler, M;Gard, S
GD VII和FA两株病毒是在实验中偶然发现的。与黄热病，是相互免疫相关，以及小鼠脑脊髓炎病毒。中枢神经系统的感染可由脑内、鼻内或腹腔内的两种菌株引起。接种。GD VII菌株通过所有3种接种方法产生的主要症状是四肢弛缓性麻痹;当通过脑内和鼻内接种产生感染时，FA菌株产生的症状可归因于脑病变。术中后。然而，接种FA株病毒，通常会产生弛缓性麻痹。病毒量为9-13 m/t。其具有2个稳定性最佳值[长划线]pH 8和pH 3.3。在37[degree]C下，1% H2 O2可使其迅速失活。在测试的有机溶剂中，乙醚没有作用，而20%乙醇在寒冷中45分钟后几乎完全灭活病毒。用（NH_4）_2SO_4沉淀病毒。随着年龄的增长，小鼠对其产生了相对的抵抗力。以及鼻内施用大的AMT。病毒。通过脑内接种相对无毒的病毒感染的小鼠对高毒力菌株产生强免疫力。研究了小鼠脑内、鼻内和腹腔内接种FA病毒株后的感染过程。
The 2 strains of virus, GD VII and FA, accidentally discovered during expts. with yellow fever, are immunologically related to each other, as well as to the virus of mouse encephalomyelitis. Infection of the central nervous system can be produced with both strains by intracerebral, intra- nasal, or intraperit. inoculations. The cardinal symptom produced by the GD VII strain by all 3 methods of inoculation is a flaccid paralysis of the limbs; the symptoms produced by the FA strain are referable to lesions of the brain when infection is produced by intracerebral and intra-nasal inoculation. Following intraperit. inoculation of the FA strain of virus, however, a flaccid paralysis is usually produced. The virus measures 9-13 m/t. It has 2 stability optima[long dash]pH 8 and pH 3.3. It is readily inactivated at 37[degree]C by 1% H2O2. Of organic solvents tested, ether had no action, whereas 20% ethyl alcohol almost completely inactivated the virus after 45 min. in the cold. The virus precipitated by (NH4)2SO4. With increasing age mice acquire a relative resistance to it. Immunity to a subsequent intracerebral inoculation can be produced by intraperit., as well as intranasal, administrations of large amts. of virus. Mice infected by the intracerebral inoculation of a relatively avirulent virus develop a strong immunity to highly virulent strains. The course of infection in mice following intracerebral, intranasal, and intraperitoneal inoculation of the FA strain of virus was studied.