Tissue specific expression and developmental regulation of two genes coding for rat fatty acid binding proteins.
Tissue specific expression and developmental regulation of two genes coding for rat fatty acid binding proteins.
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DOI：
10.1016/s0021-9258(18)89501-1
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发表时间：
1985-02
期刊：
影响因子：
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通讯作者：
J I Gordon;N. Elshourbagy;J. B. Lowe;W S Liao;D. Alpers;J. M. Taylor
中科院分区：
文献类型：
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作者：
J I Gordon;N. Elshourbagy;J. B. Lowe;W S Liao;D. Alpers;J. M. Taylor
我们研究了两个低分子量胞质脂肪酸结合蛋白的组织分布和发育调节。根据它们最初的分离位置，它们被称为肝脏和肠道脂肪酸结合蛋白(FABP)。克隆的cDNA被用来探测从各种成年大鼠组织中提取的RNA以及从胎儿、哺乳和断奶动物中获得的小肠和肝脏RNA的斑点。“肝”FABP基因在小肠和肝脏中的表达浓度最高。“肠道”FABP基因在小肠RNA中含量最高，而在肝脏中仅有微量。在胃、结肠、胰腺、脾、肺、心脏、睾丸、肾上腺和脑中均可检测到这两种RNA，其浓度为小肠的1-8%。在发育过程中，这两种mRNAs在小肠上皮中的积累都会增加。在怀孕第19天和第21天之间，首先可以检测到mRNAs。在出生后的24小时内，它们的浓度会协调增加3-4倍。此后，肠道FABP mRNA水平在哺乳期间保持不变，而肝脏FABP mRNA水平额外增加2倍。肝脏FABP mRNA水平在出生后第一天也在肝细胞中诱导，但随后在哺乳和断奶阶段不发生变化，尽管肝脏脂肪酸代谢发生了显著变化。这些观察结果支持这样的概念，即这些蛋白质的主要作用是促进脂类进入细胞和/或随后的细胞内运输和分隔化。这些数据还对胃肠外FABP的身份提出了质疑。
We have examined the tissue distribution and developmental regulation of two low molecular weight cytosolic fatty acid binding proteins. Based on their initial site of isolation, they have been referred to as liver and intestinal fatty acid binding proteins (FABP). Cloned cDNAs were used to probe blots of RNAs extracted from a wide variety of adult rat tissues as well as small intestine and liver RNA obtained from fetal, suckling, and weaning animals. The highest concentrations of “liver” FABP mRNA were found in small intestine and liver. “Intestinal” FABP mRNA is most abundant in small bowel RNA while only trace amounts were encountered in liver. Both mRNAs were detectable in stomach, colon, pancreas, spleen, lung, heart, testes, adrenal, and brain RNA at 1-8% the concentrations observed in small intestine. Accumulation of both mRNAs in the small intestinal epithelium increases during development. The mRNAs are first detectable between the 19th and 21st day of gestation. They undergo a coordinated 3-4-fold increase in concentration within the first 24 h after birth. Thereafter, gut levels of intestinal FABP mRNA remain constant during the suckling period while liver FABP mRNA increases an additional 2-fold. Liver FABP mRNA levels are also induced in hepatocytes during the first postnatal day but subsequently do not change during the suckling and weaning phase, despite marked alterations in hepatic fatty acid metabolism. These observations support the concept that the major role of these proteins is to facilitate the entry of lipids into cells and/or their subsequent intracellular transport and compartmentalization. The data also raise questions about the identity of extragastrointestinal FABPs.