Si-Wu-Tang facilitates ovarian function through improving ovarian microenvironment and angiogenesis in a mouse model of premature ovarian failure.
Si-Wu-Tang facilitates ovarian function through improving ovarian microenvironment and angiogenesis in a mouse model of premature ovarian failure.
复制标题
四物汤通过改善卵巢早衰小鼠模型的卵巢微环境和血管生成来促进卵巢功能。
DOI：
10.1016/j.jep.2021.114431
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发表时间：
2021
影响因子：
5.4
通讯作者：
Mingmin Zhang
中科院分区：
文献类型：
--
作者：
Fanru Zhou;Yufan Song;Xia Liu;Chu Zhang;Fan Li;Runan Hu;Yan;Wen;Kun;Mingmin Zhang
卵巢早衰（POF）是一种严重的疾病，其特征是过早绝经，卵巢功能明显下降，导致不孕。四物汤被医学界称为“妇科第一方”，是临床上广泛应用于调节月经周期、治疗不孕症的基本方剂。然而，SWT治疗POF的潜在作用和潜在的作用机制尚未阐明。目的本研究旨在探讨SWT治疗C57 BL/6小鼠POF的治疗作用和潜在的分子作用机制。POF模型组采用环磷酰胺（Cy，100 mg/kg）单次腹腔注射建立。SWT或脱氢表雄酮（DHEA）通过口服管饲法连续给药28天。通过激素水平、卵泡发育和血管生成的变化评价卵巢功能和病理变化。此外，生育率的统计分析也performed. ResultsSWT治疗显着改善雌激素水平，卵泡数量，抗氧化防御，和微血管形成POF小鼠。SWT可显著激活Nrf 2/HO-1和STAT 3/HIF-1α/VEGF信号通路，促进卵巢血管生成，从而改善卵巢功能。结论SWT可通过提高抗氧化能力和促进卵巢血管生成，保护卵巢功能，为SWT治疗卵巢早衰提供科学依据。
Ethnopharmacological relevancePremature ovarian failure (POF) is a severe illness, characterized by premature menopause with a markedly decrease in ovarian function, which leads to infertility. Si-Wu-Tang (SWT), also called “the first prescription of gynecology” by medical experts in China, is widely used as the basic formula in regulating the menstrual cycle and treating infertility. However, the potential effect and underlying mechanisms of action of SWT on the treatment of POF have not yet been elucidated.PurposeThis study aimed to explore the therapeutic effect and underlying molecular mechanism of action of SWT on the treatment of POF in C57BL/6 mice.Materials and methodsThe main compounds of SWT were identified by high-performance liquid chromatography (HPLC). POF model groups were established by a single intraperitoneal injection of cyclophosphamide (Cy, 100 mg/kg). SWT or dehydroepiandrosterone (DHEA) were administered via oral gavage for 28 consecutive days. Ovarian function and pathological changes were evaluated by hormone levels, follicular development, and changes in angiogenesis. Furthermore, statistical analyses of fertility were also performed.ResultsTreatment with SWT significantly improved estrogen levels, the number of follicles, antioxidant defense, and microvascular formation in POF mice. Moreover, SWT significantly activated the Nrf2/HO-1 and STAT3/HIF-1α/VEGF signaling pathways to promote angiogenesis, resulting in a better fertility outcome when compared to the model group.ConclusionsOur findings indicated that SWT protected ovarian function of Cy-induced POF mice by improving the antioxidant ability and promoting ovarian angiogenesis, thereby providing scientific evidence for the treatment of POF using SWT.