Increases in GABA concentrations during cerebral ischaemia: a microdialysis study of extracellular amino acids
Increases in GABA concentrations during cerebral ischaemia: a microdialysis study of extracellular amino acids
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DOI：
10.1136/jnnp.72.1.99
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发表时间：
2002-01-01
影响因子：
11
通讯作者：
Kirkpatrick, PJ
中科院分区：
文献类型：
--
作者：
Hutchinson, PJ;O'Connell, MT;Kirkpatrick, PJ
目的：在脑缺血患者中，兴奋性氨基酸谷氨酸和天冬氨酸的细胞外浓度增加是众所周知的。尽管来自动物研究的证据表明GABA可在缺血模型中充当神经保护剂，但较少强调抑制性氨基酸神经递质，特别是γ-氨基丁酸（GABA）的浓度。本研究的目的是研究各种兴奋性，脑损伤或蛛网膜下腔出血患者在基础条件下和脑缺血期间的抑制性和非递质氨基酸。方法：脑微透析建立在12例颅脑损伤（n=7）或蛛网膜下腔出血（n=5）。使用高效液相色谱法对总共19种（兴奋性、抑制性和非递质）氨基酸进行分析。患者在神经重症监护或动脉瘤clipping.Results：在稳定的监测期间，氨基酸的浓度是相对恒定的，使基础值建立。在6例患者中，脑缺血与GABA浓度增加（高达1350倍）有关，此外还有谷氨酸和天冬氨酸。发现谷氨酸和GABA的浓度平行增加（r=0.71，p
Objectives: Increases in the extracellular concentration of the excitatory amino acids glutamate and aspartate during cerebral ischaemia in patients are well recognised. Less emphasis has been placed on the concentrations of the inhibitory amino acid neurotransmitters, notably gamma-amino-butyric acid (GABA), despite evidence from animal studies that GABA may act as a neuroprotectant in models of ischaemia, The objective of this study was to investigate the concentrations of various excitatory, inhibitory and non-transmitter amino acids under basal conditions and during periods of cerebral ischaemia in patients with head injury or a subarachnoid haemorrhage.Methods: Cerebral microdialysis was established in 12 patients with head injury (n=7) or subarachnoid haemorrhage (n=5). Analysis was performed using high performance liquid chromatography for a total of 19 (excitatory, inhibitory and non-transmitter) amino acids. Patients were monitored in neurointensive care or during aneurysm clipping.Results: During stable periods of monitoring the concentrations of amino acids were relatively constant enabling basal values to be established. In six patients, cerebral ischaemia was associated with increases (up to 1350 fold) in the concentration of GABA, in addition to the glutamate and aspartate. Parallel increases in the concentration of glutamate and GABA were found (r=0.71, p