CLIPON: A CRISPR-Enabled Strategy that Turns Commercial Pregnancy Test Strips into General Point-of-Need Test Devices
CLIPON: A CRISPR-Enabled Strategy that Turns Commercial Pregnancy Test Strips into General Point-of-Need Test Devices
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CLIPON：一种启用 CRISPR 的策略，将商业妊娠试纸变成通用的需求测试设备
DOI：
10.1002/anie.202115907
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发表时间：
2022-02-02
影响因子：
16.6
通讯作者：
Li, Bingling
中科院分区：
文献类型：
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作者：
Tang, Yidan;Qi, Lijuan;Li, Bingling
理想的生物传感分析需要是敏感的，特异的，成本效益高的，无仪器的，和通用的。在此，我们报告了一种新的策略，称为CLIPON （CRISPR和大DNA组装诱导妊娠条带用于信号- on检测），可以提供这些特性。CLIPON将商业妊娠试纸条（PTS）集成了四种生物元素：人绒毛膜促性腺激素（hCG）、CRISPR-Cas12a、crRNA和花椰菜样大尺寸DNA组装（CLD）。CLIPON使用Cas12a/crRNA复合物来识别感兴趣的靶标并释放cld结合的hCG，以便靶标的存在可以转化为PTS上的比色信号。我们通过对HPV基因组DNA、SARS-CoV-2基因组RNA和腺苷的敏感和特异性检测，证明了CLIPON的多功能性。我们还设计了一个手机应用程序和一个手持微芯片来实现信号量化。CLIPON代表了生物传感和即时诊断的一个有吸引力的选择。
Desirable biosensing assays need to be sensitive, specific, cost-effective, instrument-free, and versatile. Herein we report a new strategy termed CLIPON (CRISPR and Large DNA assembly Induced Pregnancy strips for signal-ON detection) that can deliver these traits. CLIPON integrates a commercial pregnancy test strip (PTS) with four biological elements: the human chorionic gonadotropin (hCG), CRISPR-Cas12a, crRNA and cauliflower-like large-sized DNA assemblies (CLD). CLIPON uses the Cas12a/crRNA complex both to recognize a target of interest and to release CLD-bound hCG so that target presence can translate into a colorimetric signal on the PTS. We demonstrate the versatility of CLIPON through sensitive and specific detection of HPV genomic DNA, SARS-CoV-2 genomic RNA and adenosine. We also engineer a cell phone app and a hand-held microchip to achieve signal quantification. CLIPON represents an attractive option for biosensing and point-of-care diagnostics.