Controlling Fall armyworm (Lepidoptera:Noctuidae) Infestations in Whorl Stage Corn with Genetically Modified Bacillus thuringiensis formulations
Controlling Fall armyworm (Lepidoptera:Noctuidae) Infestations in Whorl Stage Corn with Genetically Modified Bacillus thuringiensis formulations
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DOI：
10.2307/3495579
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发表时间：
1996-09
影响因子：
1.4
通讯作者：
J. All;J. Stancil;T. Johnson;R. Gouger
中科院分区：
文献类型：
--
作者：
J. All;J. Stancil;T. Johnson;R. Gouger
利用重组DNA技术开发更有效的苏云金芽孢杆菌（柏林）（Bt）菌株可能会导致改进的杀虫产品，用于控制如秋粘虫、草地贪夜蛾（J.E. Smith），这是传统的基于BT的产品难以管理的。利用重组DNA技术从EG 2348中选育出的Bt变异株EG 1999（生物杀虫剂Condor®的活性成分），1994年，在轮生期田间玉米中，（1995年）（ECX 9526（WG））和颗粒（ECX 9526（G）制剂）与Javelin®（WG），一种衍生自天然存在的Bt菌株的杀虫剂，和灭多威（Lannate® LV）。1994年，在室内用秋粘虫测定了Bt重组蛋白和天然蛋白对1龄幼虫的残留毒力，结果表明，Bt重组蛋白和天然蛋白对1龄幼虫的残留毒力均小于48 h。1994年，将刚孵化的FAW置于A形框架笼中的植物上，以评估这是一种建立人工田间侵染的方法，用于当自然FAW种群较低时使用。笼内的植物损害和杀虫剂控制与笼外的严重自然侵扰相似。
Development of more potent strains of Bacillus thuringiensis (Berliner) (Bt) using recombinant DNA technology may lead to improved insecticidal products for controlling pests like the fall armyworm, Spodoptera frugiperda (J. E. Smith), which have been difficult to manage with conventional Bt-based products. EG1999, a variant Bt strain developed by recombinant DNA technology from EG2348 (the active ingredient of the bioinsecticide Condor®), showed improved control of FAW infestations in whorl stage field corn in 1994 (oil flowable and granular formulations) and sweetcorn in 1995 [wettable granule (ECX9526) (WG)] and granular [ECX9526 (G) formulations] as compared with Javelin® (WG), an insecticide derived from a naturally occurring Bt strain, and methomyl (Lannate® LV). In 1994, leaf samples taken 1 h, 1, 2, 3, 5, and 7 d after spraying and assayed in the laboratory with fall armyworm showed that the Bt products (recombinant and natural) had less than 48 h residual toxicity to 1st instar larvae. In 1994 freshly hatched FAW were placed on plants in A-frame cages to evaluate this as a method to establish artificial field infestations for use when natural FAW populations are low. The plant damage and insecticide control in the cages were similar to the heavy natural infestations outside the cages.