ROLE OF OVARIAN HORMONES IN THE LONG-TERM CONTROL OF GLUCOSE-HOMEOSTASIS - EFFECTS ON INSULIN-SECRETION
ROLE OF OVARIAN HORMONES IN THE LONG-TERM CONTROL OF GLUCOSE-HOMEOSTASIS - EFFECTS ON INSULIN-SECRETION
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DOI：
10.1007/bf00281829
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发表时间：
1980-01-01
期刊：
影响因子：
8.2
通讯作者：
AHMEDSOROUR, H
中科院分区：
文献类型：
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作者：
BAILEY, CJ;AHMEDSOROUR, H
研究人员检查了卵巢激素在长期控制小鼠 B 细胞功能中的作用。切除卵巢的成年雌性小鼠每天接受皮下注射治疗。雌二醇（5μg/kg）、黄体酮（1mg/kg）、两种激素组合或载体的替代剂量仅持续15周。与对照组小鼠相比，卵巢切除导致葡萄糖耐量试验期间血浆葡萄糖浓度增加 40%，血浆胰岛素对葡萄糖（2 g/kg i.p.）的反应降低 26%，血浆胰岛素对精氨酸（2 g/kg i.p.）的反应降低 32%。当来自卵巢切除小鼠的胰岛在含有 28 mmol/l 葡萄糖或 2.8 mmol/l 葡萄糖和 5 mmol/l 精氨酸的培养基中孵育 30 分钟时，胰岛素释放分别减少了 23% 和 31%。切除卵巢的小鼠的胰腺和胰岛总胰岛素含量分别减少了 36%，B 细胞数量减少了 39%。雌二醇和黄体酮治疗部分或完全预防了卵巢切除术的这些有害影响。结果表明，卵巢雌激素和孕激素可能在雌性小鼠 B 细胞能力的长期维持中发挥重要作用。
The role of ovarian hormones in the long-term control of B-cell function in the mouse was examined. Ovariectomized adult female mice were treated with daily s.c. replacement doses of estradiol (5 .mu.g/kg), progesterone (1 mg/kg), both hormones combined or vehicle only for 15 wk. Ovariectomy caused a 40% increase in plasma glucose concentrations during glucose tolerance tests, a 26% decrease in the plasma insulin response to glucose (2 g/kg i.p.) and a 32% decrease in the plasma insulin response to arginine (2 g/kg i.p.) compared with control mice. When islets from ovariectomized mice were incubated for 30 min in media containing 28 mmol/l glucose or 2.8 mmol/l glucose with 5 mmol/l arginine, insulin release was reduced by 23 and 31%, respectively. Total pancreatic and islet insulin content were each decreased by 36% and the number of B-cells was decreased by 39% in the ovariectomized mice. These detrimental effects of ovariectomy were partially or totally prevented by the estradiol and progesterone treatments. The results indicate that ovarian estrogens and progestogens may play an important role in the long-term maintenance of B-cell competence in the female mouse.