A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development.
A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development.
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DOI：
10.1038/sdata.2015.46
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发表时间：
2015
期刊：
影响因子：
9.8
通讯作者：
Burns R
中科院分区：
文献类型：
--
作者：
Harris KM;Spacek J;Bell ME;Parker PH;Lindsey LF;Baden AD;Vogelstein JT;Burns R
对突触电路和可塑性的重新关注强调了连续切片电子显微镜（3DEM）的3D重建的重要性。取成年大鼠海马CA 1区神经元，在50-60 nm厚的连续切片上，以~ 2nm的X-Y分辨率成像。这是第一个密集重建的海马体积。所有轴突、树突、神经胶质和突触均重建为围绕大树突棘的立方体（约10 μm3）、围绕倾斜树突段（3.4 μm长）的圆柱体（约43 μm3）和围绕顶端树突段（4.9 μm长）的平行六面体（约178 μm3）。这些数据提供了用于识别3DEM中的超微结构对象的标准，用于建模突触传递的生物物理特性的逼真重建，以及用于增强重建工具的测试床。代表性的突触定量从不同的截面，微管，多聚核糖体，滑面内质网，和内体被确定和重建的树突的一个子集。原始图像、迹线和重建软件和文件可在Open Connectome Project（数据引文1）中免费获得和可视化。
Resurgent interest in synaptic circuitry and plasticity has emphasized the importance of 3D reconstruction from serial section electron microscopy (3DEM). Three volumes of hippocampal CA1 neuropil from adult rat were imaged at X-Y resolution of ~2 nm on serial sections of ~50–60 nm thickness. These are the first densely reconstructed hippocampal volumes. All axons, dendrites, glia, and synapses were reconstructed in a cube (~10 μm3) surrounding a large dendritic spine, a cylinder (~43 μm3) surrounding an oblique dendritic segment (3.4 μm long), and a parallelepiped (~178 μm3) surrounding an apical dendritic segment (4.9 μm long). The data provide standards for identifying ultrastructural objects in 3DEM, realistic reconstructions for modeling biophysical properties of synaptic transmission, and a test bed for enhancing reconstruction tools. Representative synapses are quantified from varying section planes, and microtubules, polyribosomes, smooth endoplasmic reticulum, and endosomes are identified and reconstructed in a subset of dendrites. The original images, traces, and Reconstruct software and files are freely available and visualized at the Open Connectome Project (Data Citation 1).