Complete sequence of the mitochondrial genome of Tetrahymena thermophila and comparative methods for identifying highly divergent genes
Complete sequence of the mitochondrial genome of Tetrahymena thermophila and comparative methods for identifying highly divergent genes
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DOI：
10.1093/nar/gkg270
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发表时间：
2003-03-15
影响因子：
14.9
通讯作者：
Li, JL
中科院分区：
文献类型：
--
作者：
Brunk, CF;Lee, LC;Li, JL
嗜热四膜虫线粒体基因组的完整序列已被确定，并与梨形四膜虫线粒体基因组进行了比较。序列相似性清楚地表明整个 T.thermophila 和 T.pyriformis 线粒体基因组的同源性。 T.thermophila 基因组非常紧凑，大多数基因间区域都很短（只有 3 个基因间区域长于 63 bp），仅占基因组的 3.8%。 nad9 基因在 T.thermophila 中串联复制。长末端反向重复序列和 nad9 基因正在经历协同进化。有 55 个假定基因：3 个核糖体 RNA 基因、8 个转移 RNA 基因、22 个具有假定指定功能的蛋白质和 22 个功能未知的附加开放阅读框。为了将同源性的指示扩展到氨基酸序列相似性之外，我们检查了线粒体蛋白的许多物理化学特性，包括理论等电点、分子量，特别是预测的跨膜跨越区域。这种方法使我们能够识别 ymf58 (nad4L)、ymf62 (nad6) 和 ymf60 (rpl6) 的同源物。
The complete sequence of the mitochondrial genome of Tetrahymena thermophila has been determined and compared with the mitochondrial genome of Tetrahymena pyriformis. The sequence similarity clearly indicates homology of the entire T.thermophila and T.pyriformis mitochondrial genomes. The T.thermophila genome is very compact, most of the intergenic regions are short (only three are longer than 63 bp) and comprise only 3.8% of the genome. The nad9 gene is tandemly duplicated in T.thermophila. Long terminal inverted repeats and the nad9 genes are undergoing concerted evolution. There are 55 putative genes: three ribosomal RNA genes, eight transfer RNA genes, 22 proteins with putatively assigned functions and 22 additional open reading frames of unknown function. In order to extend indications of homology beyond amino acid sequence similarity we have examined a number of physico-chemical properties of the mitochondrial proteins, including theoretical pI, molecular weight and particularly the predicted transmembrane spanning regions. This approach has allowed us to identify homologs to ymf58 (nad4L), ymf62 (nad6) and ymf60 (rpl6).