Bilayered PLGA/Wool Keratin Composite Membranes Support Periodontal Regeneration in Beagle Dogs.
Bilayered PLGA/Wool Keratin Composite Membranes Support Periodontal Regeneration in Beagle Dogs.
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DOI：
10.1021/acsbiomaterials.6b00357
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发表时间：
2016-10
影响因子：
5.8
通讯作者：
Hualin Zhang;Juan Wang;Hairong Ma;Yueli Zhou;Xuerong Ma;Jinsong Liu;Jin Huang;N. Yu
中科院分区：
文献类型：
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作者：
Hualin Zhang;Juan Wang;Hairong Ma;Yueli Zhou;Xuerong Ma;Jinsong Liu;Jin Huang;N. Yu
使用引导组织再生膜的再生程序目前在牙周治疗中得到了很好的应用。本研究采用溶剂浇铸和静电纺丝的方法制备了聚乳酸-乙醇酸共聚物(PLGA)/羊毛角蛋白(WK)双层复合膜。然后，将复合膜用于Beagle犬引导组织再生的效果评价。结果表明，双层PLGA/羊毛角蛋白复合膜的底层为致密的PLGA/羊毛角蛋白复合膜，上层为疏松、多孔、三维的纤维状PLGA/羊毛角蛋白复合膜(羊毛角蛋白含量为0、0.5、1、2、4wt%)。羊毛角蛋白的加入显著提高了复合材料的力学性能。双层PLGA/1.0%羊毛角蛋白复合膜具有最佳的极限强度和杨氏模量值。PLGA/羊毛角蛋白双层复合膜均表现出较高的热稳定性和热氧化稳定性。GTR结果表明，PLGA/1.0%羊毛角蛋白复合膜在12周后能有效促进牙周组织的再生，其效果与胶原膜相当。因此，双层PLGA/羊毛角蛋白复合膜显示出很大的潜力来满足GTR膜的需求。为进一步研究PLGA/羊毛角蛋白复合膜在牙周组织中的应用和牙周病的治疗奠定了基础。
Regenerative procedures using guided tissue regeneration (GTR) membrane are presently well-established in periodontal therapy. In this study, bilayered poly(lactic-co-glycolic acid) (PLGA)/wool keratin (WK) composite membranes were prepared for use as GTR membrane by the solvent casting and electrospinning methods. Then, the composite membranes were used to evaluate the effects in guided tissue regeneration in beagle dogs. The results showed that the bottom layer (casting film) of the bilayered PLGA/wool keratin composite membranes was a compact PLGA/wool keratin membrane, and the upper layer (electrospun film) was a loose, porous, three-dimensional, and fibrous PLGA/wool keratin membrane (The wool keratin was at five different levels: 0, 0.5, 1, 2, or 4 wt %). The mechanical properties of the composites were significantly enhanced by the addition of wool keratin. The bilayered PLGA/1.0% wool keratin composite membranes presented the best values of ultimate strength and Young's modulus. All of the bilayered PLGA/wool keratin composite membranes showed high thermal and thermooxidative stabilities. The GTR results showed that the PLGA/1.0% wool keratin composite membranes could effectively promote the periodontal tissue regeneration after 12 weeks, and have a similar effect to the collagen membrane on the regeneration of the periodontal tissue. Thus, the bilayered PLGA/wool keratin composite membranes show great potential to meet the demand for GTR membrane. The study will serve as a foundation for further study of the PLGA/wool keratin membranes for GTR application and the therapy of periodontal disease.