In vitro inhibition of Plasmodium falciparum merozoite invasion by human plasma fibronectin
In vitro inhibition of Plasmodium falciparum merozoite invasion by human plasma fibronectin
复制标题
人血浆纤连蛋白体外抑制恶性疟原虫裂殖子侵袭
DOI：
10.1017/s0031182000050939
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发表时间：
1985
期刊：
影响因子：
2.4
通讯作者：
A. Capron
中科院分区：
文献类型：
--
作者：
M. Ouaissi;E. Dutoit;A. Vernes;S. Guerret;A. Capron
通过使用兔抗人纤连蛋白的抗血清进行间接免疫荧光测定，测试了从柠檬酸盐血液中分离的人纤连蛋白与恶性疟原虫结合的能力。在裂殖体感染的红细胞内的裂殖子上观察到阳性反应。在非寄生红细胞上没有观察到结合。使用体外抑制试验进一步研究了人纤连蛋白对恶性疟原虫生长的影响；约 100 μg/ml 的人纤连蛋白可抑制寄生虫增殖 50%。低于 10 μg/ml 时观察到轻微抑制。讨论了这一发现的意义。
Human fibronectin isolated from citrated blood was tested for its ability to bind to Plasmodium falciparum by an indirect immunofluorescent assay using rabbit antiserum to human fibronectin. A positive reaction was observed on merozoites inside schizont-infected erythrocytes. The binding was not observed on non-parasitized red blood cells. The effect of human fibronectin on P.falciparum growth was further studied using an in vitro inhibition assay; 50% inhibition of parasite multiplication was obtained with approximately 100 μg/ml of human fibronectin. Slight inhibition was observed below 10 μg/ml. The significance of this finding is discussed.