Dehydration-induced expression of LEA proteins in an anhydrobiotic chironomid
Dehydration-induced expression of LEA proteins in an anhydrobiotic chironomid
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DOI：
10.1016/j.bbrc.2006.07.003
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发表时间：
2006-09-15
影响因子：
3.1
通讯作者：
Okuda, Takashi
中科院分区：
文献类型：
--
作者：
Kikawada, Takahiro;Nakahara, Yuichi;Okuda, Takashi
晚期胚胎发育丰富（莱亚）蛋白与脱水生物的脱水耐受性有关。非洲摇蚊的幼虫，Polypedilum vanderplanki，能够承受几乎完全干燥，在此期间，他们进入假死状态。在这里，我们开发了一个EST数据库，从脱水幼虫和分离的三个cDNA编码蛋白质（PvLEA1，PvLEA2，和PvLEA3）具有高度显着的匹配组3莱亚蛋白。所有三个例子的mRNA和蛋白质水平都因干燥或高盐度施加的脱水应激而增加，并且一种蛋白质PvLEA 2可能会在翻译后加工成更小的分子。首次描述节肢动物中的莱亚蛋白质基因表明，该蛋白质家族广泛分布于无脊椎动物门，并且动物、植物和微生物具有类似的对抗脱水应激的机制。(c)2006年爱思唯尔公司All rights reserved.
Late embryogenesis abundant (LEA) proteins are associated with desiccation tolerance in anhydrobiotic organisms. The larvae of an African chironomid, Polypedilum vanderplanki, are able to withstand almost complete desiccation during which they enter a state of suspended animation. Here, we developed an EST database from desiccating larvae and isolated three cDNAs encoding proteins (PvLEA1, PvLEA2, and PvLEA3) with highly significant matches to Group 3 LEA proteins. Both mRNA and protein levels of all three examples were increased by dehydration stress imposed by either desiccation or hypersalinity, and one protein, PvLEA2, is likely to be post-translationally processed into smaller molecules. This first description of LEA protein genes in arthropods suggests that this protein family is widespread throughout invertebrate phyla, and that animals, plants, and microorganisms possess similar mechanisms for combating dehydration stress. (c) 2006 Elsevier Inc. All rights reserved.