Proteomic Landscape Has Revealed Small Rubber Particles Are Crucial Rubber Biosynthetic Machines for Ethylene-Stimulation in Natural Rubber Production
Proteomic Landscape Has Revealed Small Rubber Particles Are Crucial Rubber Biosynthetic Machines for Ethylene-Stimulation in Natural Rubber Production
复制标题
蛋白质组学景观揭示了小橡胶颗粒是天然橡胶生产中乙烯刺激的关键橡胶生物合成机器
DOI：
10.3390/ijms20205082
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发表时间：
2019-10-02
影响因子：
5.6
通讯作者：
Wang, Xuchu
中科院分区：
文献类型：
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作者：
Wang, Dan;Xie, Quanliang;Wang, Xuchu
橡胶颗粒是天然橡胶生物合成和储存的特殊细胞器。乙烯可以通过增加小橡胶颗粒（SRPs）的产生，调节蛋白质积累和激活许多酶的活性来显着提高胶乳产量。我们进行了定量蛋白质组学研究不同SRPs乙烯刺激后的差异凝胶电泳（DIGE）和使用同量异位素标签的相对和绝对定量（iTRAQ）方法。在DIGE中，79个差异积累蛋白（DAP）被确定为乙烯响应蛋白。我们的研究结果表明，大量的NRB相关蛋白已急剧诱导乙烯刺激后。其中，23个蛋白被鉴定为橡胶延伸因子（REF）和小橡胶颗粒蛋白（SRPP）家族成员，包括16个REF和7个SRPP异构体。然后，从64个REF/SRPP家族成员中鉴定了138个独特的磷酸化肽，包含129个磷酸化氨基酸，并且大多数丝氨酸和苏氨酸被磷酸化。此外，我们通过iTRAQ从超过2000种SRP蛋白中鉴定了226种DAP。综合分析表明，几乎所有的NRB相关蛋白都可以在SRPs中检测到，并且许多蛋白对乙烯刺激具有正响应。这些结果表明，乙烯可能通过调节一些关键蛋白质的积累来刺激胶乳的产生。REF和SRPP异构体的磷酸化修饰可能对NRB至关重要，SRP可能充当复杂的天然橡胶生物合成机器。
Rubber particles are a specific organelle for natural rubber biosynthesis (NRB) and storage. Ethylene can significantly improve rubber latex production by increasing the generation of small rubber particles (SRPs), regulating protein accumulation, and activating many enzyme activities. We conducted a quantitative proteomics study of different SRPs upon ethylene stimulation by differential in-gel electrophoresis (DIGE) and using isobaric tags for relative and absolute quantification (iTRAQ) methods. In DIGE, 79 differentially accumulated proteins (DAPs) were determined as ethylene responsive proteins. Our results show that the abundance of many NRB-related proteins has been sharply induced upon ethylene stimulation. Among them, 23 proteins were identified as rubber elongation factor (REF) and small rubber particle protein (SRPP) family members, including 16 REF and 7 SRPP isoforms. Then, 138 unique phosphorylated peptides, containing 129 phosphorylated amino acids from the 64 REF/SRPP family members, were identified, and most serine and threonine were phosphorylated. Furthermore, we identified 226 DAPs from more than 2000 SRP proteins by iTRAQ. Integrative analysis revealed that almost all NRB-related proteins can be detected in SRPs, and many proteins are positively responsive to ethylene stimulation. These results indicate that ethylene may stimulate latex production by regulating the accumulation of some key proteins. The phosphorylation modification of REF and SRPP isoforms might be crucial for NRB, and SRP may act as a complex natural rubber biosynthetic machine.