Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis.
Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis.
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DOI：
10.3109/1354750x.2015.1105497
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Bay-Jensen AC
中科院分区：
文献类型：
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作者：
Kjelgaard-Petersen C;Siebuhr AS;Christiansen T;Ladel C;Karsdal M;Bay-Jensen AC
目的：表征测量炎症性骨关节炎滑膜细胞外基质周转的生物标志物。 研究方法：通过条件培养基中的C1 M、C3 M和acMMP 3评估用各种细胞因子和生长因子处理的人原代成纤维细胞样滑膜细胞和滑膜外植体（SME）。 结果如下：TNFα使SME中的C1 M显著增加7倍（p = 0.0002），C3 M增加24倍（p = 0.0011），acMMP 3增加14倍（p < 0.0001）。IL-1β还使SME中的C1 M增加了5倍（p = 0.00094），C3 M增加了4倍（p = 0.007），acMMP 3增加了18倍（p < 0.0001）。 结论：生物标志物C1 M、C3 M和acMMP-3是离体滑膜炎生物标志物，并与SME模型一起提供了转化工具。
Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNFα significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p < 0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p < 0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.