Two Different Homing Pathways Involving Integrin β7 and E-selectin Significantly Influence Trafficking of CD4 Cells to the Genital Tract Following Chlamydia muridarum Infection
Two Different Homing Pathways Involving Integrin β7 and E-selectin Significantly Influence Trafficking of CD4 Cells to the Genital Tract Following Chlamydia muridarum Infection
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DOI：
10.1111/j.1600-0897.2009.00704.x
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发表时间：
2009-06-01
影响因子：
3.6
通讯作者：
Darville, Toni
中科院分区：
文献类型：
--
作者：
Kelly, Kathleen A.;Chan, Ann M.;Darville, Toni
沙眼衣原体在世界范围内导致性传播感染和生殖功能障碍，这是抗生素无法预防的。通过对整合素β7-/-小鼠或内皮细胞上缺乏E-选择素的小鼠的荧光CD4+T细胞的过继转移研究，检测了小鼠体内CD4+T细胞对衣原体感染生殖道(GT)组织的穿透能力。小鼠体内迁移研究表明，缺乏α4β7或E-选择素显著减少了CD4T细胞向感染衣原体的小鼠生殖道组织的转运。在感染沙眼衣原体的生殖道组织中，CD4+T细胞至少使用两种不同的黏附机制，涉及粘膜归巢途径的整合和选择素途径的积聚。
Chlamydia trachomatis causes STI and reproductive dysfunction worldwide which is not preventable with antibiotics. Identifying a population of endocervical T cells to target in vaccine development would enhance efficacy.Trafficking of murine CD4+ lymphocytes to Chlamydia muridarum infected genital tract (GT) tissue in vivo was measured using adoptive transfer studies of fluorescent CD4+ T cells from integrin beta 7-/- mice or mice which lack E-selectin on endothelial cells.Murine in vivo migration studies showed that lack of alpha 4 beta 7 or E-selectin significantly reduced trafficking of CD4 T cells to the GT of mice infected with C. muridarum.CD4+ T cells use at least two different adhesive mechanisms involving an integrin of the mucosal homing pathway and selectin pathway to accumulate in the GT during C. muridarum infection.