A new complement function: solubilization of antigen-antibody aggregates.
A new complement function: solubilization of antigen-antibody aggregates.
复制标题
新的补体功能：抗原抗体聚集体的溶解。
DOI：
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发表时间：
1975
影响因子：
11.1
通讯作者：
V. Nussenzweig
中科院分区：
文献类型：
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作者：
G. Miller;V. Nussenzweig
抗原抗体聚集体在 37 度下与新鲜血清一起孵育时溶解，产生相对较小分子量的免疫复合物，其中含有抗原、抗体和补体 (C3) 决定簇。溶解是补体依赖性的，需要游离 Mg++ 但不需要 Ca++，并且在 C4 或 C5 缺陷动物的血清中进行。正常或缺乏 C4 的豚鼠血清中存在 Ca++ 时，该过程会加速，表明备解素系统的 Cl 旁路激活参与其中。免疫沉淀物也可以通过针对抗原-抗体晶格中包含的抗体分子决定簇的抗体单价片段（Fab）来溶解。类似地，有人提出，补体介导的溶解可能是由补体片段与免疫聚集体中的抗体的组合诱导的。
Antigen-antibody aggregates are solubilized when incubated with fresh serum at 37 degrees, yielding immune-complexes of relatively small molecular weight which contain antigen, antibody, and complement (C3)determinants. Solubilization is complement-dependent,requires free Mg++ but not Ca++, and proceeds in sera from C4- or C5-deficient animals. It is accelerated in the presence of Ca++ in normal or C4-deficient guinea pig serum, suggesting involvement of the Cl-bypass activation of the properdin system. Immune precipitates can also be solubilized by monovalent fragments (Fab) of antibodies directed against determinants of the antibody molecules included in the antigen-antibody lattice. Similarly, it is suggested that complement-mediated solubilization might be induced by the combination of a complement fragment with the antibody in the immune-aggregate.