Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu
Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu
复制标题
中国小麦地方品种黄芳珠抗赤霉病数量性状位点
DOI：
10.1007/s10681-012-0631-2
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发表时间：
2012-05-01
期刊：
影响因子：
1.9
通讯作者：
Gu, Shiliang
中科院分区：
文献类型：
--
作者：
Li, Tao;Bai, Guihua;Gu, Shiliang
我国小麦地方品种黄方株（HFZ）对赤霉病（FHB）具有较高的抗性。为了确定HFZ对FHB抗性的染色体区域，利用HFZ与美国硬春小麦Wheaton杂交获得了f8重组自交系（RIL）。在温室和田间环境下，采用单小花接种法对FHB进行了评价。鉴定出两个主要效应的数量性状位点（QTL）。1个QTL位于3B染色体短臂上，解释了35.4%的表型变异；另一个QTL分配给7AL，解释了18.0%的FHB应答表型变异。此外，通过单标记回归在1AS、1B和5AS染色体上检测到3个次要QTL。HFZ贡献了所有有利等位基因。在温室和田间环境中，在3BS和7AL上QTL上有HFZ等位基因的RIL的侵染小穗率显著低于没有这些等位基因的RIL。HFZ组合了先前报道的种质中的几个等位基因，是提高小麦抗FHB能力的有希望的替代来源。
The Chinese wheat landrace Huangfangzhu (HFZ) has a high level of resistance to Fusarium head blight (FHB). To identify chromosomal regions that are responsible for FHB resistance in HFZ, F8recombinant inbred lines (RIL) were developed from a cross between HFZ and Wheaton, a U.S. hard spring wheat. FHB was evaluated by single floret inoculation in both greenhouse and field environments. Two quantitative trait loci (QTL) with major effects were identified. One QTL was located on the short arm of chromosome 3B, and explained 35.4% of the phenotypic variation; the other QTL was assigned to 7AL and explained 18.0% of the phenotypic variation for FHB response. In addition, three minor QTL were detected on chromosomes 1AS, 1B and 5AS by single marker regression. HFZ contributed all favorable alleles. The RIL with HFZ alleles at the QTL on 3BS and 7AL displayed significantly lower percentages of infected spikelets than RIL without these alleles in both greenhouse and field environments. HFZ combined several alleles from germplasm reported previously and is a promising alternative source for improving wheat FHB resistance.