Advanced glycation alters expression of the 67kDa laminin receptor in retinal microvascular endothelial cells
Advanced glycation alters expression of the 67kDa laminin receptor in retinal microvascular endothelial cells
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DOI：
10.1016/s0024-3205(01)01084-0
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发表时间：
2001-05-04
期刊：
影响因子：
6.1
通讯作者：
Stitt, AW
中科院分区：
文献类型：
--
作者：
McKenna, DJ;Nelson, J;Stitt, AW
67kDa 层粘连蛋白受体 (67LR) 在血管细胞功能和功能障碍中发挥重要作用。本研究检测了暴露于 AGE 后视网膜微血管内皮细胞中 67LR 的表达。将视网膜微血管内皮细胞暴露于AGE-BSA，或在甲基乙二醛修饰的层粘连蛋白或Matrigel (TM) 上生长，并通过Western Blotting和RT-PCR/Southern blotting分析67LR的表达。质膜蛋白质印迹和 RT-PCR/Southern 印迹显示暴露于 AGE 后 67LR 蛋白/mRNA 表达显着上调 (p
The 67kDa laminin receptor (67LR) plays an important role in vascular cell function and dysfunction. The present study has examined 67LR expression in retinal microvascular endothelial cells after exposure to AGEs. Retinal microvascular endothelial cells were exposed to either AGE-BSA, or were grown on methylglyoxal-modified laminin or Matrigel (TM) and expression of 67LR analysed by Western Blotting and RT-PCR/Southern blotting. Western blotting of plasma membrane and RT-PCR/Southern blotting revealed a significant upregulation of 67LR protein/mRNA expression after exposure to AGEs (p