Identification of agronomically important QTL in tetraploid potato cultivars using a marker-trait association analysis
Identification of agronomically important QTL in tetraploid potato cultivars using a marker-trait association analysis
复制标题
DOI：
10.1007/s00122-013-2254-y
复制
发表时间：
2014-03-01
影响因子：
5.4
通讯作者：
van Eck, Herman J.
中科院分区：
文献类型：
--
作者：
D'hoop, Bjorn B.;Keizer, Paul L. C.;van Eck, Herman J.
马铃薯19个块茎品质性状在205个栽培品种和299个育种无性系中进行了表型分析。利用3364个AFLP位点和653个SSR等位基因进行关联分析，确定了这些性状的QTL。第一组包括205个历史和当代的四倍体马铃薯栽培品种，这些栽培品种在两个地点的田间试验中进行了两次重复（学术组）。第二组由299个马铃薯品种组成，包括从五家荷兰马铃薯育种公司获得的最新品种和参考品种（工业组）。第二组的表型数据在各个育种公司的后续克隆选择世代期间收集。对19个农艺性状和品质性状进行了QTL定位。使用了两种关联映射模型：基线模型，没有，和一个更先进的模型与人口结构和遗传相关性的校正。校正群体结构和遗传相关性进行了估计从标记信息的亲属关系矩阵。检测到的QTL不仅部分证实了以前的研究，如块茎形状和油炸颜色，但也发现了新的QTL，如烘烤后变黑和酶促布朗宁。多个QTL表现出多效性效应。
Key message Nineteen tuber quality traits in potato were phenotyped in 205 cultivars and 299 breeder clones. Association analysis using 3364 AFLP loci and 653 SSR-alleles identified QTL for these traits.Two association mapping panels were analysed for marker-trait associations to identify quantitative trait loci (QTL). The first panel comprised 205 historical and contemporary tetraploid potato cultivars that were phenotyped in field trials at two locations with two replicates (the academic panel). The second panel consisted of 299 potato cultivars and included recent breeds obtained from five Dutch potato breeding companies and reference cultivars (the industrial panel). Phenotypic data for the second panel were collected during subsequent clonal selection generations at the individual breeding companies. QTL were identified for 19 agro-morphological and quality traits. Two association mapping models were used: a baseline model without, and a more advanced model with correction for population structure and genetic relatedness. Correction for population structure and genetic relatedness was performed with a kinship matrix estimated from marker information. The detected QTL partly not only confirmed previous studies, e.g. for tuber shape and frying colour, but also new QTL were found like for after baking darkening and enzymatic browning. Pleiotropic effects could be discerned for several QTL.