QUANTITATIVE INVESTIGATIONS OF ADHESIVENESS OF CIRCULATING POLYMORPHONUCLEAR LEUKOCYTES TO BLOOD-VESSEL WALLS
QUANTITATIVE INVESTIGATIONS OF ADHESIVENESS OF CIRCULATING POLYMORPHONUCLEAR LEUKOCYTES TO BLOOD-VESSEL WALLS
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DOI：
10.1113/jphysiol.1972.sp009808
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发表时间：
1972-01-01
影响因子：
5.5
通讯作者：
BORN, GVR
中科院分区：
文献类型：
--
作者：
ATHERTON, A;BORN, GVR
1.本文介绍了一种新的简单方法，用于定量循环中多形核白细胞或粒细胞对血管壁的粘附。制备麻醉仓鼠的颊囊或麻醉小鼠的小部分肠系膜，用于连续显微镜观察选定的小静脉。当粒细胞滚动通过血管一侧的选定点时，对移动足够慢以单独可见的粒细胞计数1或2分钟。这些细胞的速度分布是通过分析电影。通过观察二磷酸腺苷离子导入引起的血小板血栓栓塞，用胶片测量微静脉中的平均血流速度。通过在染色的组织切片的标准区域中计数粒细胞来定量粒细胞向组织中的迁移.在仓鼠颊囊小静脉的对照实验中，滚动粒细胞计数通常在制备后不久达到最大值，然后降至较低的恒定值。在小鼠肠系膜小静脉中，计数从开始时保持在低的近似恒定值至少3小时。3.微静脉中的平均血流速度在900 μ/sec和200 μ/sec之间。所有滚动的粒细胞移动得慢得多;在仓鼠颊囊小静脉中，平均速度约为20 μ/秒，在小鼠肠系膜小静脉中约为10 μ/秒。在这些平均值附近，单个细胞的速度分布较窄.通过灌流乙二胺四乙酸盐（EDTA，0·1 M）可以消除粒细胞的滚动，这表明这种现象依赖于钙或镁离子。将药物局部应用于观察到的小静脉。高浓度的人血清白蛋白、胰蛋白酶或组胺对滚动粒细胞计数无影响.应用对粒细胞有趋化作用的哈马斯顿酪蛋白或大肠杆菌滤液，滚动粒细胞计数增加。这些药物未引起观察到的小静脉中平均血流速度的改变，这可能是对滚动粒细胞计数的影响。当E.通过小鼠肠道灌注coliculture滤液，引流静脉血中滚动粒细胞计数的增加与滤液浓度的对数成正比.局部应用血浆球蛋白渗透因子或淋巴结渗透因子也可增加滚动粒细胞计数.标准组织学切片中的粒细胞计数在对照制剂中未显示显著增加，但在应用Hammarsten酪蛋白后显著增加。
1. A new simple method is described for quantitating the adhesiveness of circulating polymorphonuclear leucocytes, or granulocytes, to the walls of blood vessels. The cheek pouch of anaesthetized hamsters or a small part of the mesentery of anaesthetized mice were prepared for continuous microscopic observation of selected venules. Those granulocytes which moved sufficiently slowly to be individually visible were counted for 1 or 2 min periods as they rolled past a selected point on one side of a vessel. The velocity distribution of these cells was determined by analysing films. Films were used also to measure mean blood flow velocity in the venules by observing embolizing platelet thrombi induced by the iontophoretic application of adenosine diphosphate. Emigration of granulocytes into the tissues was quantitated by enumerating them in standard areas of stained histological sections.2. In control experiments with hamster cheek pouch venules, the rolling granulocyte count usually passed through a maximum shortly after the preparation was set up and then fell to a low constant value. In mouse mesentery venules the count remained at a low approximately constant value from the beginning for at least 3 hr.3. The mean velocity of blood flow in the venules was between 900 and 200 μ/sec. All rolling granulocytes moved much more slowly; in hamster cheek pouch venules the mean velocity was about 20 μ/sec and in mouse mesentery venules about 10 μ/sec. Around these means the velocity distribution of individual cells was narrow.4. Rolling of granulocytes was abolished by superfusing ethylenediamine tetra‐acetate (EDTA, 0·1M) suggesting that the phenomenon depends on calcium or magnesium ions.5. Agents were applied locally to the observed venules. Human serum albumin, trypsin or histamine in high concentrations did not affect the rolling granulocyte count.6. The rolling granulocyte count was increased during the application of Hammarsten casein orEscherichia coliculture filtrate which are chemotactic to granulocytesin vitro. These agents did not cause alterations in mean blood flow velocity in the observed venules which might have accounted for the effect on the rolling granulocyte counts. WhenE. coliculture filtrate was perfused through mouse intestine the increase in rolling granulocyte count in the draining venous blood was proportional to the logarithm of the concentration of filtrate.7. The rolling granulocyte count was also increased by the local application of plasma globulin permeability factor or lymph node permeability factor.8. Granulocyte counts in standard histological sections showed no significant increases in control preparations but considerable increases following the application of Hammarsten casein.