TRANSPLANTATION OF ORGAN CULTURES OF ADULT HUMAN SKIN
TRANSPLANTATION OF ORGAN CULTURES OF ADULT HUMAN SKIN
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DOI：
10.1111/1523-1747.ep12260178
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发表时间：
1970-01-01
影响因子：
6.5
通讯作者：
KARASEK, M
中科院分区：
文献类型：
--
作者：
SUMMERLIN, WT;CHARLTON, E;KARASEK, M
在标准组织培养生长条件下，裂层和全层皮肤外植体在器官培养中保持活力长达 6 周。在此期间，表皮变薄，基底膜变厚。真皮中弹性蛋白的含量减少。 Epiboly 在培养的第 2 至第 4 周完成。当移植到全层伤口部位时，表皮结构恢复正常。然而，真皮弹性蛋白不会被重新合成。在一年半的时间里对移植皮肤进行的后续研究中，没有发现真皮或表皮细胞异常生长的证据。
Under standard tissue culture growth conditions, both split-thickness and full-thickness skin explants remain viable for up to 6 weeks in organ culture. During this period the epidermis becomes thin, and the basement membrane thickens. The content of elastin in the dermis decreases. Epiboly is complete by the 2nd to the 4th week in culture. When transplanted to a full-thickness wound site the epidermal architecture returns to normal. Dermal elastin is, however, not resynthesized. No evidence for an abnormal growth of cells in either the dermis or the epidermis has been detected in follow-up studies of the grafted skin over a one and one-half year interval.