Design, Preparation, and Characterization of Peptide-Based Nanocarrier for Gene Delivery
Design, Preparation, and Characterization of Peptide-Based Nanocarrier for Gene Delivery
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DOI：
10.1007/978-1-4939-9516-5_5
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Hosseinkhani, Saman
中科院分区：
文献类型：
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作者：
Alipour, Mohsen;Hosseinkhani, Saman
由于存在各种细胞内外屏障和需要传递载体，将核酸输送到真核细胞是具有挑战性的。然而，目前的基因传递载体，包括PLL、PEI和脂质体，都存在着纳米载体联合毒性、效率低、多分散性和不可生物降解性等问题。在这里，我们描述了我们开发安全、有效和单分散的多肽载体用于基因传递的策略。我们解释了利用基因工程技术将不同的功能基序整合到一个小于100 nm的单一多肽中，这有助于将基因输送到哺乳动物细胞。
The delivery of nucleic acid to eukaryotic cells is challenging due to presence of various intra- and extracellular barriers and need to delivery carriers. However, current gene delivery carriers, including PLL, PEI, and liposome, suffer from nanocarrier associate toxicity, low efficiency and polydispersity, and non-biodegradability. Here we describe our strategy for developing safe, efficacious, and monodisperse peptide-based carrier for gene delivery. We explain the use of genetic engineering technology for integrating various functional motifs in a single peptide, with less than 100 nm size, which facilitated gene delivery into mammalian cell.