Microwave-assisted extraction of cyclotides from Viola ignobilis
Microwave-assisted extraction of cyclotides from Viola ignobilis
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DOI：
10.1016/j.ab.2015.12.001
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发表时间：
2016-03-15
影响因子：
2.9
通讯作者：
Ghassempour, Alireza
中科院分区：
文献类型：
--
作者：
Farhadpour, Mohsen;Hashempour, Hossein;Ghassempour, Alireza
环肽是一个有趣的环状植物肽家族。它们独特的三维结构，包括头尾相连的圆形主链和三个二硫键，赋予它们对热、化学和酶降解的稳定性。它们在极端条件下独特的稳定性使人们想到可以使用微波辅助提取 (MAE) 等严酷的提取方法而不影响其结构。 MAE 已被引入作为提取天然化合物的有效提取方法，但很少用于肽和蛋白质提取。在这项工作中，微波辐射被应用于环肽的提取。该程序在不同条件下使用微波仪器分多个步骤进行。高效液相色谱 (HPLC) 和基质辅助激光解吸/电离飞行时间 (MALDI-TOF) 结果显示环肽结构在微波辐射下的稳定性。对影响 MAE 效力的影响参数（包括时间、温度和溶剂比例）进行了优化。最佳条件是照射时间为 20 分钟、系统功率为 1200 W、温度为 60 摄氏度、甲醇/水比例为 90:10 (v/v) 作为溶剂。 MAE 结果与浸渍提取的比较表明，环肽序列和提取率之间存在相似性。 (C) 2015 Elsevier Inc. 保留所有权利。
Cyclotides are an interesting family of circular plant peptides. Their unique three-dimensional structure, comprising a head-to-tail circular backbone chain and three disulfide bonds, confers them stability against thermal, chemical, and enzymatic degradation. Their unique stability under extreme conditions creates an idea about the possibility of using harsh extraction methods such as microwave-assisted extraction (MAE) without affecting their structures. MAE has been introduced as a potent extraction method for extraction of natural compounds, but it is seldom used for peptide and protein extraction. In this work, microwave irradiation was applied to the extraction of cyclotides. The procedure was performed in various steps using a microwave instrument under different conditions. High-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) results show stability of cyclotide structures on microwave radiation. The influential parameters, including time, temperature, and the ratio of solvents that are affecting the MAE potency, were optimized. Optimal conditions were obtained at 20 min of irradiation time, 1200 W of system power in 60 degrees C, and methanol/water at the ratio of 90:10 (v/v) as solvent. The comparison of MAE results with maceration extraction shows that there are similarities between cyclotide sequences and extraction yields. (C) 2015 Elsevier Inc. All rights reserved.