Microinjection of DNA into Aedes triseriatus ova and detection of integration.

Microinjection of DNA into Aedes triseriatus ova and detection of integration.
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DOI：
10.4269/ajtmh.1988.39.502
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发表时间：
1988-11
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
通讯作者：
V. McGrane;J. Carlson;B. Miller;B. Beaty
V. McGrane;J. Carlson;B. Miller;B. Beaty
中科院分区：
其他
文献类型：
--
作者：
V. McGrane;J. Carlson;B. Miller;B. Beaty

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建立了三色伊蚊显微注射转化技术。如果干燥并在绒毛膜硬化之前接种蚊卵，可以很容易地用大约0.9NL的接种剂显微注射蚊卵。卵子被注射了赋予抗生素(G418)对幼虫抗药性的分子结构。Southern印迹分析表明，抗性结构插入到蚊子基因组中。
A technique was developed for microinjection and transformation of Aedes triseriatus mosquitoes. Mosquito ova can be readily microinjected with approximately 0.9 nl of inoculum if desiccated and inoculated before hardening of the chorion. Ova were injected with molecular constructs that conferred antibiotic (G418) resistance to larvae. Insertion of the antibiotic resistance construct into the mosquito genome was demonstrated by Southern blot analysis.