Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts
Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts
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DOI：
10.1016/j.foodchem.2010.11.115
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发表时间：
2011-06-01
期刊：
影响因子：
8.8
通讯作者：
Pushpa, K. K.
中科院分区：
文献类型：
--
作者：
Roja, G.;Vikrant, B. H.;Pushpa, K. K.
当地的药用植物。阿达达木(L)Nees.(棘科)，俗称Vasaka，用于治疗过敏原引起的支气管阻塞、哮喘和结核病，并具有保护肝脏的活性。该植物是维生素C的天然来源。在Murashige和Skoog的附加各种植物生长调节剂的培养基上开始的血管紧张素组织培养，显示出生物碱、血管紧张素和血管紧张素酮的存在。新梢培养物水提物中含有较高水平的这些生物碱。这些提取物中的血管紧张素和血管紧张素含量分别为干重的5.98%和5.2%，精选亲本植物的水提取物中血管紧张素的干重为3-4%。亲本植物和新梢培养物的甲醇提取物在血管紧张素和血管紧张素的含量上都有数量上的差异。水提物对DPPH自由基有最大清除活性。(C)2010爱思唯尔有限公司。保留所有权利。
The indigenous medicinal plant. Adhatoda vasica (L) Nees. (Acanthaceae), commonly known as vasaka, is used as a herbal remedy for allergen-induced bronchial obstruction, asthma, and tuberculosis and possesses hepatoprotective activity. This plant is a natural source of vitamin C. Tissue cultures of A. vasica, initiated on Murashige and Skoog's medium supplemented with various plant growth regulators, showed the presence of alkaloids, vasicine and vasicinone. Water extracts of shoot cultures contained high levels of these alkaloids. The vasicine and vasicinone contents in these extracts were 5.98% and 5.2% of dry weight and the water extracts of the selected elite parent plant contained 3-4% dry weight of vasicine. The methanolic extracts of the parent plant and shoot cultures showed quantitative differences in the level of both vasicine and vasicinone. Maximum free radical-scavenging activity of DPPH radicals was observed in the water extracts. (C) 2010 Elsevier Ltd. All rights reserved.