Water dynamics in MCF-7 breast cancer cells: a neutron scattering descriptive study
Water dynamics in MCF-7 breast cancer cells: a neutron scattering descriptive study
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DOI：
10.1038/s41598-019-45056-8
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发表时间：
2019-06-18
影响因子：
4.6
通讯作者：
Bordallo, Heloisa N.
中科院分区：
文献类型：
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作者：
Martins, Murillo L.;Dinitzen, Alexander B.;Bordallo, Heloisa N.
癌细胞中的水流动性可能是预测肿瘤进展或缓解的有力参数。在目前的描述性工作中，通过结合中子散射和热分析，实现了对这一概念的新认识。这些结果为解开水动力学在乳腺癌细胞（MCF-7）中所起的作用迈出了第一步。通过热分析，细胞被探测为体积状和承压水种群的微米水库。从这个角度来看，我们发现药物明显改变了承压水的性质。我们已经独立地验证了这一想法，通过使用非弹性中子散射访问细胞水动力学。最后，准弹性中子散射数据的分析使我们能够假设，在这个特定的细胞系中，响应于药物在纳秒时间尺度上的作用，细胞内水的扩散增加。
Water mobility in cancer cells could be a powerful parameter to predict the progression or remission of tumors. In the present descriptive work, new insight into this concept was achieved by combining neutron scattering and thermal analyses. The results provide the first step to untangle the role played by water dynamics in breast cancer cells (MCF-7) after treatment with a chemotherapy drug. By thermal analyses, the cells were probed as micrometric reservoirs of bulk-like and confined water populations. Under this perspective we showed that the drug clearly alters the properties of the confined water. We have independently validated this idea by accessing the cellular water dynamics using inelastic neutron scattering. Finally, analysis of the quasi-elastic neutron scattering data allows us to hypothesize that, in this particular cell line, diffusion increases in the intracellular water in response to the action of the drug on the nanosecond timescale.