DETERMINATION OF PROLIFERATION INDEX IN ADVANCED OVARIAN-CANCER USING QUANTITATIVE IMAGE-ANALYSIS
DETERMINATION OF PROLIFERATION INDEX IN ADVANCED OVARIAN-CANCER USING QUANTITATIVE IMAGE-ANALYSIS
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DOI：
10.1093/ajcp/99.6.736
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发表时间：
1993-06-01
影响因子：
3.5
通讯作者：
BERCHUCK, A
中科院分区：
文献类型：
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作者：
JORDAN, PA;KERNS, BJM;BERCHUCK, A
已有研究表明，单抗Ki-67与一种仅由增殖细胞表达的核抗原发生反应。探讨应用图像分析技术定量检测卵巢上皮癌Ki-67染色(增殖指数[PI])的可行性。对50例晚期卵巢癌患者进行了PI测定。冰冻切片用Ki-67单抗进行免疫组织化学染色，通过静态图像分析计算PI。在35例III期卵巢癌中，PI中位数为8.9%，而在15例IV期卵巢癌中PI中位数为17.7%(P=0.06)。PI与组织学分级无关。Ki-67高表达(≥7.5%)的32例患者的中位生存期为16.8个月，差异有统计学意义(P
It has been shown that the monoclonal antibody Ki-67 reacts with a nuclear antigen that is expressed only by proliferating cells. The feasibility of using image analysis to quantitate Ki-67 staining (proliferation index [PI]) of epithelial ovarian cancers was investigated. The PI was determined in 50 advanced-stage primary ovarian cancers. Frozen sections were immunostained with the Ki-67 monoclonal antibody, and the PI was calculated using static image analysis. Among 35 stage III ovarian carcinomas, the median PI was 8.9%, compared with 17.7% in 15 stage IV cancers (P = 0.06). There was no relationship between PI and histologic grade. The median survival time of 32 patients whose cancers had a high Ki-67 expression (greater-than-or-equal-to 7.5%) was 16.8 months, which differed significantly (P