Environmental doses of arsenic exposure are associated with increased reproductive-age male urinary hormone excretion and in vitro Leydig cell steroidogenesis.
Environmental doses of arsenic exposure are associated with increased reproductive-age male urinary hormone excretion and in vitro Leydig cell steroidogenesis.
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DOI：
10.1016/j.jhazmat.2020.124904
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发表时间：
2020-12
影响因子：
13.6
通讯作者：
Meiping Tian;Yi-Xin Wang;Xiaofei Wang;Heng Wang;Liangpo Liu;Jie Zhang;Bingru Nan;Heqing Shen
中科院分区：
文献类型：
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作者：
Meiping Tian;Yi-Xin Wang;Xiaofei Wang;Heng Wang;Liangpo Liu;Jie Zhang;Bingru Nan;Heqing Shen
人类普遍暴露于多种来源的砷，慢性砷暴露可能与男性生殖健康有关。虽然砷暴露与血液中性激素分泌的相关性已有报道，但缺乏尿中性激素排泄的研究。尿性激素排泄已成为评估性腺功能的补充策略。在此，我们确定了砷环境暴露与尿性激素消除和体外Leydig细胞类固醇生成之间的关系。对451名育龄男性重复尿砷、睾酮（T）、雌二醇（E2）和孕酮（P）进行了测定。此外，体外Leydig细胞MLTC-1类固醇合成实验旨在模拟低人类暴露的真实世界场景。多元线性回归模型被用来评估尿砷水平与尿激素的关系。尿砷浓度与尿性激素（T，E2和P）水平呈正相关。Anin体外试验进一步证明，以人群为基础的环境暴露范围（0.01-5 μM）的砷诱导Leydig细胞类固醇生成的效力。我们的研究结果表明，低剂量砷暴露具有内分泌干扰作用，刺激睾丸间质细胞类固醇生成和加速尿类固醇排泄，这扩展了以前的知识，高剂量砷暴露与性类固醇的生产，被认为是抗雄激素的负相关。
Humans are ubiquitously exposed to arsenic from multiple sources, and chronic arsenic exposure may be associated with male reproductive health. Although association regarding arsenic exposure and sex hormone secretion in blood has been reported, sex hormone excretion in urine studies is lacking. Urinary sex hormone excretion has emerged as a complementary strategy to evaluate gonadal function. Herein, we determined the associations between environmental exposure to arsenic and urinary sex hormone elimination andin vitroLeydig cell steroidogenesis. Concentrations of arsenic and testosterone (T), estradiol (E2) and progesterone (P) in repeated urine samples were determined among 451 reproductive-age males. Moreover, anin vitroLeydig cell MLTC-1 steroidogenesis experiment was designed to simulate real-world scenarios of low human exposure. Multivariable linear regression models were used to assess the associations of urinary arsenic levels with urinary hormones. Urinary arsenic concentrations were positively associated with urinary sex hormone (T, E2, and P) levels. Anin vitrotest further demonstrated that a population-based environmental exposure range (0.01–5 μM) of arsenic induced Leydig cell steroidogenesis potency. Our results indicate that low-dose arsenic exposure exhibits an endocrine disrupting effect by stimulating Leydig cell steroidogenesis and accelerating urinary steroid excretion, which extends previous knowledge of the inverse association of high-dose arsenic exposure with sexual steroid production that is assumed to be anti-androgen.